Gamma2A Jak2 Cell Line
The Gamma2A Jak2 Cell Line lacks inducible phosphorylation of the IFN-gamma receptor 1 (R1) subunit, JAK1, and STAT1 and IFN-gamma-inducible cell surface.
Contributors
| Inventor | Institute |
|---|---|
| George Stark ; Ian Kerr | Cancer Research UK, London Research Institute: Lincoln's Inn Fields |
| Cat. #: | 151681 |
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| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Sarcoma |
| Research Fields: | Cell signaling and signal transduction;Immunology |
| Organism: | Human |
| Model: | Transgenic |
| Cancer Types In Detail: | Fibrosarcoma |
| Product description: | The Gamma2A Jak2 Cell Line has been developed by expressing protein tyrosine kinase JAK2 in a mutant cell line defective in the interferon-gamma signal transduction pathway. By using this cell line, the structural and functional analysis of JAk2 in IFN-gamma signalling has been examined. The cell line revealed that the specificity of JAK may lie mainly in their structural interaction with different receptors and signalling proteins rather than in the substrate specificity of their kinase dom… |
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| Production details: | The human sarcoma cell line HT 1080 was transfected with a vector encoding a selectable marker (guanine phosphoribosyltransferase) regulated by gamma interferon to create the parent 2C4- gamma 2A cell line. This cell line was then co-transfected with expression construct coding for JAK2. |
| Cellosaurus ID: | CVCL_1D51 |
| Parental cell line: | HT 1080 |
| disease: | Cancer |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | DMEM with 10% v/v heat-inactivated FCS and 400ug/ml G418 plus 0.5 ug/ml puromycin |
| Recommended controls: | HT 1080 parental line |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
Bonjardim et al. 1998. Braz J Med Biol Res. 31(11):1389-95. PMID: 9921273. JAK/STAT-deficient cell lines. Kohlhuber et al. 1997. Mol Cell Biol. 17(2):695-706. PMID: 9001223. A JAK1/JAK2 chimera can sustain alpha and gamma interferon responses. Watling et al. 1993. Nature. 366(6451):166-70. PMID: 7901766. Complementation by the protein tyrosine kinase JAK2 of a mutant cell line defective in the interferon-gamma signal transduction pathway. |
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