Gamma2A Jak2 Cell Line
- Name George Stark ; Ian Kerr
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool name: Gamma2A Jak2 Cell Line
Tool type: Cell Lines
Tool sub-type: Continuous
Parental cell line: HT 1080
Cancer type: Fibrosarcoma
Description: The Gamma2A Jak2 Cell Line has been developed by expressing protein tyrosine kinase JAK2 in a mutant cell line defective in the interferon-gamma signal transduction pathway. By using this cell line, the structural and functional analysis of JAk2 in IFN-gamma signalling has been examined. The cell line revealed that the specificity of JAK may lie mainly in their structural interaction with different receptors and signalling proteins rather than in the substrate specificity of their kinase domains.
Research area: Cell Signaling & Signal Transduction; Immunology
Production details: The human sarcoma cell line HT 1080 was transfected with a vector encoding a selectable marker (guanine phosphoribosyltransferase) regulated by gamma interferon to create the parent 2C4- gamma 2A cell line. This cell line was then co-transfected with expression construct coding for JAK2.
Cellosaurus ID: CVCL_1D51
- For Research Use Only
- • Bonjardim et al. 1998. Braz J Med Biol Res. 31(11):1389-95. PMID: 9921273.
- • JAK/STAT-deficient cell lines.
- • Kohlhuber et al. 1997. Mol Cell Biol. 17(2):695-706. PMID: 9001223.
- • A JAK1/JAK2 chimera can sustain alpha and gamma interferon responses.
- • Watling et al. 1993. Nature. 366(6451):166-70. PMID: 7901766.
- • Complementation by the protein tyrosine kinase JAK2 of a mutant cell line defective in the interferon-gamma signal transduction pathway.