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Contributor Information

  • Name George Stark ; Ian Kerr
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: Gamma2A Jak2 Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: HT 1080
  • Organism: Human
  • Cancer type: Fibrosarcoma
  • Disease: Cancer
  • Model: Transgenic
  • Description: The Gamma2A Jak2 Cell Line has been developed by expressing protein tyrosine kinase JAK2 in a mutant cell line defective in the interferon-gamma signal transduction pathway. By using this cell line, the structural and functional analysis of JAk2 in IFN-gamma signalling has been examined. The cell line revealed that the specificity of JAK may lie mainly in their structural interaction with different receptors and signalling proteins rather than in the substrate specificity of their kinase domains.
  • Research area: Cell Signaling & Signal Transduction; Immunology
  • Production details: The human sarcoma cell line HT 1080 was transfected with a vector encoding a selectable marker (guanine phosphoribosyltransferase) regulated by gamma interferon to create the parent 2C4- gamma 2A cell line. This cell line was then co-transfected with expression construct coding for JAK2.
  • Cellosaurus ID: CVCL_1D51

  • For Research Use Only

Target Details

  • Target: Gamma2A Jak2

Application Details

Handling

  • Format: Frozen
  • Growth medium: DMEM with 10% v/v heat-inactivated FCS and 400ug/ml G418 plus 0.5 ug/ml puromycin
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

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  • Available on request

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References

  • •  Bonjardim et al. 1998. Braz J Med Biol Res. 31(11):1389-95. PMID: 9921273.
  • •  JAK/STAT-deficient cell lines.
  • •  Kohlhuber et al. 1997. Mol Cell Biol. 17(2):695-706. PMID: 9001223.
  • •  A JAK1/JAK2 chimera can sustain alpha and gamma interferon responses.
  • •  Watling et al. 1993. Nature. 366(6451):166-70. PMID: 7901766.
  • •  Complementation by the protein tyrosine kinase JAK2 of a mutant cell line defective in the interferon-gamma signal transduction pathway.