CELL LINES
Contributor Information
- Name Jonathon Pines
- Institute University of Cambridge
Tool Details
- Tool name: hTERT-RPE1 mRuby-APC15 Cell Line
- Tool type: Cell Lines
- Parental cell line: hTERT-RPE1
- Organism: Human
- Tissue: Eye
- Model: Transgenic
- Conditional: Yes
- Conditional description: Tetracycline inducible
- Description: The hTERT-RPE1 mRuby-APC15 Cell Line is a tet-inducible APC15 reporter cell line. APC15 is a component of the human cell cycle Anaphase promoting complex/cyclosome (APC/C). It is required for progression from metaphase during cell cycle. The uncharacterised open reading frame C11orf51 has been identified in a systematic proteomic analysis of APC/C purified from HeLa cell extracts. Human C11orf51 is conserved in vertebrates and invertebrates and has homology to Schizosaccharomyces pombe APC15 (PM gene ID: 2540655), and Schizosaccharomyces cerevisiae Mnd2 (PM gene ID: 854843).
- Research area: Cell biology
- Production details: APC15 was amplified by PCR from a full length cDNA clone (ORFeome clone 100066673). Tetracycline inducible cell lines expressing APC15 IRES2 mRuby were generated using the FLIP in system and a modified pCDNA5/FRT/TO vector. The parental tetracycline inducible RPE cell line was created by random integration of an FRT site and a Tet repressor gene into hTERT RPE1 cells followed by selection of a clone that showed high levels of gene expression repression in the absence of tetracycline.
- Cellosaurus ID: CVCL_5F68
- For Research Use Only
Target Details
- Target: APC15
Application Details
Handling
- Format: Frozen
- Growth medium: F12/DMEM (1:1) media supplemented with 10% FCS, sodium bicarbonate, glutamate and penicillin/streptavidin.
- Shipping conditions: Dry ice
Documentation
Related Tools
References
- • Mansfield et al. 2011. Nat Cell Biol. 13:1234-43. PMID: 21926987
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