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Contributor Information

  • Name Julia Reichelt
  • Institute Newcastle University

Tool Details

  • Tool name: GFP-KSC4 Keratinocyte Stem Cell Line
  • Tool type: Cell Lines
  • Organism: Mouse
  • Disease: Dermatology
  • Growth properties: Adherent
  • Model: Transgenic
  • Description: The GFP-KSC4 Keratinocyte Stem Cell line can be used for the generation of in vitro epidermal â?‚€?‚˜skin equivalentsâ?‚€?‚™ to be used as screening tools to look at the activity of NCEâ?‚€?‚™s and/or NBEâ?‚€?‚™s of dermatological interest, and/or to screen for such NCE and/or NBE in high throughput systems. It may also be of use to non-pharmaceutical healthcare and/or cosmetic companies involved in the personal vitality and/or beauty product development segments. Murine keratinocyte stem cell line.
  • Research area: Drug development; Stem cell biology
  • Production details: Keratinocytes were isolated from neonatal homozygous C57BL/6-Tg(CAG-EGFP)1Osb/J mouse skin. The cell line was established by growing cells for the first 8 passages in co-culture with 3T3-J2 fibroblast feeder cells. From passage 9 onwards cells were grown without feeder cells.
  • Cellosaurus ID: CVCL_W142

  • For Research Use Only

Target Details

  • Target: GFP-KSC4

Application Details

Handling

  • Format: Frozen
  • Growth medium: See remarks...
  • Shipping conditions: Dry ice

Documentation

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References

  • •  Reichelt and Haase. 2010. Methods Mol Biol 585:59-69
  • •  PMID: 19907996 Okabe et al. 1997 FEBS Letters 407:313-9. PMID: 9175875 HĂ?›her T et al., 2011. Stem Cell Rev. PMID: 21874280 Vollmers A et al., 2011. PMID: 21892602