GFP-KSC4 Keratinocyte Stem Cell Line
- Name Julia Reichelt
- Institute Newcastle University
Tool name: GFP-KSC4 Keratinocyte Stem Cell Line
Tool type: Cell Lines
Growth properties: Adherent
Description: The GFP-KSC4 Keratinocyte Stem Cell line can be used for the generation of in vitro epidermal ÄË?Â?Âskin equivalentsÄË?Â?Â to be used as screening tools to look at the activity of NCEÄË?Â?Âs and/or NBEÄË?Â?Âs of dermatological interest, and/or to screen for such NCE and/or NBE in high throughput systems. It may also be of use to non-pharmaceutical healthcare and/or cosmetic companies involved in the personal vitality and/or beauty product development segments. Murine keratinocyte stem cell line.
Research area: Drug development; Stem cell biology
Production details: Keratinocytes were isolated from neonatal homozygous C57BL/6-Tg(CAG-EGFP)1Osb/J mouse skin. The cell line was established by growing cells for the first 8 passages in co-culture with 3T3-J2 fibroblast feeder cells. From passage 9 onwards cells were grown without feeder cells.
Cellosaurus ID: CVCL_W142
- For Research Use Only
- • Reichelt and Haase. 2010. Methods Mol Biol 585:59-69
- • PMID: 19907996
Okabe et al. 1997 FEBS Letters 407:313-9. PMID: 9175875
HÄ?her T et al., 2011. Stem Cell Rev. PMID: 21874280
Vollmers A et al., 2011. PMID: 21892602