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Contributor Information

  • Name Julia Reichelt
  • Institute Newcastle University

Tool Details

  • Tool name: GFP-KSC4 Keratinocyte Stem Cell Line
  • Tool type: Cell Lines
  • Organism: Mouse
  • Disease: Dermatology
  • Growth properties: Adherent
  • Model: Transgenic
  • Description: The GFP-KSC4 Keratinocyte Stem Cell line can be used for the generation of in vitro epidermal ‘skin equivalents’ to be used as screening tools to look at the activity of NCE’s and/or NBE’s of dermatological interest, and/or to screen for such NCE and/or NBE in high throughput systems. It may also be of use to non-pharmaceutical healthcare and/or cosmetic companies involved in the personal vitality and/or beauty product development segments. Murine keratinocyte stem cell line.
  • Research area: Drug Discovery & Development ; Stem Cell Biology
  • Production details: Keratinocytes were isolated from neonatal homozygous C57BL/6-Tg(CAG-EGFP)1Osb/J mouse skin. The cell line was established by growing cells for the first 8 passages in co-culture with 3T3-J2 fibroblast feeder cells. From passage 9 onwards cells were grown without feeder cells.
  • Cellosaurus ID: CVCL_W142

  • For Research Use Only

Target Details

  • Target: GFP-KSC4

Application Details

Handling

  • Format: Frozen
  • Growth medium: See remarks...
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Reichelt and Haase. 2010. Methods Mol Biol 585:59-69
  •   PMID: 19907996 Okabe et al. 1997 FEBS Letters 407:313-9. PMID: 9175875 HĂśher T et al., 2011. Stem Cell Rev. PMID: 21874280 Vollmers A et al., 2011. PMID: 21892602