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Contributor Information

  • Name Sharon Tooze
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: MEF ULK1 KO (SIM) Cell Line
  • Tool type: Cell Lines
  • Parental cell line:
  • Organism: Mouse
  • Tissue: Embryo
  • Disease: Cancer, neurodegeneration, infectious disease
  • Description: The MEF ULK1 KO (SIM) cell line can be used to study ULK1-dependent processes, including autophagy. A more complete phenotype requires depletion of ULK2 by RNAi . Mouse embryonic fibroblasts derived from a ULK1 homozygous knock out mouse embryo and immortalized by serial passaging (spontaneous transformation).
  • Research area: Cancer ; Apoptosis and Programmed Cell Death ; Cell Signaling & Signal Transduction ; Metabolism ; Neurobiology

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: DMEM + 20% FCS + 2mM Glutamine + pen/strep
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.
  •   Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.
  •   Chan et al. 2009. Mol Cell Biol. 29(1):157-71. PMID: 18936157.
  •   Kinase-inactivated ULK proteins inhibit autophagy via their conserved C-terminal domains using an Atg13-independent mechanism.