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Contributor Information

  • Name Sharon Tooze
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: MEF ULK1 KO (SIM) Cell Line
  • Tool type: Cell Lines
  • Organism: Mouse
  • Tissue: Embryo
  • Disease: Cancer; Neurodegeneration; Infectious disease
  • Growth properties: Autophagy, fibroblast
  • Model: Knock-Out
  • Description: The MEF ULK1 KO (SIM) cell line can be used to study ULK1-dependent processes, including autophagy. A more complete phenotype requires depletion of ULK2 by RNAi . Mouse embryonic fibroblasts derived from a ULK1 homozygous knock out mouse embryo and immortalized by serial passaging (spontaneous transformation).
  • Research area: Cancer; Apoptosis and autophagy; Cell signaling and signal transduction; Metabolism; Neurobiology
  • Production details: Primary embryonic fibroblasts were isolated from the embryos of a pregnant female Ulk1-/- mouse at day 13p.c. The MEFs were immortalised by SIM using a standard serial passaging protocol.
  • Cellosaurus ID: CVCL_5A52

  • For Research Use Only

Target Details

  • Target: ULK1

Application Details

Handling

  • Format: Frozen
  • Growth medium: DMEM + 20% FCS + 2mM Glutamine + pen/strep
  • Shipping conditions: Dry ice

Documentation

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References

  • •  McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.
  • •  Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.
  • •  Chan et al. 2009. Mol Cell Biol. 29(1):157-71. PMID: 18936157.
  • •  Kinase-inactivated ULK proteins inhibit autophagy via their conserved C-terminal domains using an Atg13-independent mechanism.