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Contributor Information

  • Name Sharon Tooze
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: HEK293-A GFP-WIPI2 Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: HEK293-A
  • Organism: Human
  • Tissue: Embryo
  • Disease: Cancer, neurodegeneration
  • Growth properties: Autophagy
  • Model: Transgenic
  • Description: The HEK293-A GFP-WIPI2 cell line can be used to study autophagy by light microscopy.It is also useful for high throughput microscopy screens. Stable cell line expressing GFP-tagged human WIPI2 in background of HEK293-A cell line
  • Research area: Cancer ; Apoptosis and Programmed Cell Death ; Cancer ; Cell Signaling & Signal Transduction ; Cellular Transport ; Drug Discovery & Development ; Metabolism
  • Production details: HEK293-A cells were transfected with GFP-WIPI2 then selcted with G418, and stable, amino acid responsive clones were selected.
  • Cellosaurus ID: CVCL_5A21
  • Additional notes: HEK293 cells were obtained under licence from AdVec

  • For Research Use Only

Target Details

  • Target: Human WIPI2, GFP

Application Details

Handling

  • Format: Frozen
  • Growth medium: DMEM with 10% FCS
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   Dooley et al. 2014. Mol Cell. 55(2):238-52. PMID: 24954904.
  •   WIPI2 links LC3 conjugation with PI3P, autophagosome formation, and pathogen clearance by recruiting Atg12-5-16L1.