CELL LINES
Contributor Information
- Name Walter Bodmer
- Institute University of Oxford
Tool Details
- Tool name: C75 Colorectal adenocarcinoma Cell Line
- Tool type: Cell Lines
- Organism: Human
- Tissue: Colon
- Cancer type: Adenocarcnoma of the sigmoid colon
- Disease: Cancer
- Model: Tumour line
- Conditional: Yes
- Description: The C75 cell line was established from a 56-year old male patient with a moderately well differentiated adenocarcnoma of the sigmoid colon classified as Dukes' stage D
- Research area: Cancer; Drug Discovery & Development
- Cellosaurus ID: CVCL_5248
- For Research Use Only
Target Details
Application Details
Handling
- Format: Frozen
- Growth medium: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cmË using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Culture medium: Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
- Shipping conditions: Dry ice
Documentation
- Available on request
Related Tools
References
- • Browning et al. 1993. Proc Natl Acad Sci U S A. 90(7):2842-5. PMID: 8464898.
- • Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.
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