CELL LINES
Contributor Information
- Name Walter Bodmer
- Institute University of Oxford
Tool Details
- Tool name: C75 Colorectal adenocarcinoma Cell Line
- Tool type: Cell Lines
- Organism: Human
- Tissue: Colon
- Cancer type: Digestive / Gastrointestinal cancer
- Disease: Cancer
- Model: Tumour line
- Conditional: Yes
- Description: The C75 cell line was established from a 56-year old male patient with a moderately well differentiated adenocarcnoma of the sigmoid colon classified as Dukes' stage D
- Research area: Cancer; Drug development
- Cellosaurus ID: CVCL_5248
- For Research Use Only
Target Details
Application Details
Handling
- Format: Frozen
- Growth medium: Iscove′s Modified Dulbecco′s Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
- Temperature: 37C
- Atmosphere: 5% CO2
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Subculture routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37C. The cells grow very slowly - following resuscitation or subculture the cells take at least 48 hours
Documentation
Related Tools
References
- • Browning et al. 1993. Proc Natl Acad Sci U S A. 90(7):2842-5. PMID: 8464898.
- • Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.
.svg)
Unit size: 
.svg)
.svg)
