CELL LINES
Contributor Information
- Name Walter Bodmer
- Institute University of Oxford
Tool Details
- Tool name: C99 Colorectal adenocarcinoma Cell Line
- Tool type: Cell Lines
- Organism: Human
- Tissue: Colon
- Cancer type: Adenocarcinoma
- Disease: Cancer
- Model: Tumour line
- Conditional: Yes
- Description: The C99 cell line was established from a 69-year old male patient with a moderately well differentiated adenocarcinoma of the rectum classified as Dukes' stage C
- Research area: Cancer; Drug development
- Cellosaurus ID: CVCL_8176
- For Research Use Only
Target Details
Application Details
Handling
- Format: Frozen
- Growth medium: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm? using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37?° C. culture medium: Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
- Shipping conditions: Dry ice
Documentation
Related Tools
References
- • Efstathiou et al. 1999. Proc Natl Acad Sci U S A. 96(5):2316-21. PMID: 10051639.
- • Mutated epithelial cadherin is associated with increased tumorigenicity and loss of adhesion and of responsiveness to the motogenic trefoil factor 2 in colon carcinoma cells.
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