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Contributor Information

  • Name Walter Bodmer
  • Institute University of Oxford

Tool Details

  • Tool name: C99 Colorectal adenocarcinoma Cell Line
  • Tool type: Cell Lines
  • Parental cell line:
  • Organism: Human
  • Tissue: Colon
  • Cancer type: Adenocarcinoma
  • Disease: Cancer
  • Conditional: Yes
  • Description: The C99 cell line was established from a 69-year old male patient with a moderately well differentiated adenocarcinoma of the rectum classified as Dukes' stage C
  • Research area: Cancer ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37° C. culture medium: Iscove's Modified Dulbecco's Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Efstathiou et al. 1999. Proc Natl Acad Sci U S A. 96(5):2316-21. PMID: 10051639.
  •   Mutated epithelial cadherin is associated with increased tumorigenicity and loss of adhesion and of responsiveness to the motogenic trefoil factor 2 in colon carcinoma cells.