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Contributor Information

  • Name Walter Bodmer
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: C125PM Colorectal Cell Line
  • Tool type: Cell Lines
  • Parental cell line:
  • Organism: Human
  • Tissue: Colon
  • Cancer type: Adenocarcinoma
  • Disease: Cancer
  • Conditional: Yes
  • Description: C125PM is a human colorectal adenocarcinoma cell line.
  • Research area: Cancer ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C.
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Rowan et al. 2000. Proc Natl Acad Sci U S A. 97(7):3352-7. PMID: 10737795.
  •   APC mutations in sporadic colorectal tumors: A mutational "hotspot" and interdependence of the "two hits".