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Contributor Information

  • Name Sharon Tooze
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: MEF ULK1 ULK2 DKO (SIM) Cell Line
  • Tool type: Cell Lines
  • Organism: Mouse
  • Tissue: Embryo
  • Disease: Cancer; Neurodegeneration; Infectious disease
  • Growth properties: Fibroblast
  • Model: Knock-Out
  • Conditional: Yes
  • Description: The MEF ULK1 ULK2 DKO (SIM) cell line provides a tool for the study of Ulk1 and Ulk2 and of Autophagy. Mouse embryonic fibroblast Ulk1/Ulk2 double knock-out cell line is from an embryo with a mixed genetic background (Blk6/129 Agouti).
  • Research area: Cancer; Apoptosis and Programmed Cell Death; Cell Signaling & Signal Transduction; Metabolism; Neurobiology
  • Production details: Primary embryonic fibroblasts were isolated from the embryos of a pregnant female Ulk1-/- Ulk2-/+ mouse at day 13 p.c. The embryos were genotyped to identify those that were Ulk1 -/- Ulk2 -/- and the MEFs that were isolated and cultured were immortalised by SIM using a standard serial passaging protocol.
  • Cellosaurus ID: CVCL_5A56

  • For Research Use Only

Target Details

  • Target: ULK1, ULK2

Application Details

Handling

  • Format: Frozen
  • Growth medium: DMEM + 20% FCS + 2mM Glutamine + pen/strep
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.
  •   Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.