CELL LINES
Contributor Information
- Name Sharon Tooze
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: MEF ULK2 KO (SIM) Cell Line
- Tool type: Cell Lines
- Organism: Mouse
- Tissue: Embryo
- Disease: Cancer; Neurodegeneration; Infectious disease
- Growth properties: Fibroblast
- Model: Knock-Out
- Description: The MEF ULK2 KO (SIM) cell line provides a tool for the study of Ulk2 and of Autophagy and has a comparable genetic background to MEF Ulk1 Ulk2 DKO and MEF Ulk1 KO cell lines. Mouse embryonic fibroblast Ulk2 knock-out cell line is derived from an embryo with a mixed genetic background (Blk6/129B Agouti) similar to MEF Ulk1 Ulk2 DKO cell line.
- Research area: Cancer; Apoptosis and autophagy; Cell signaling and signal transduction; Metabolism; Neurobiology
- Production details: Primary embryonic fibroblasts were isolated from the embryos of a pregnant female mouse at day 13p.c. The embryos were genotyped to identify those that were Ulk1 +/+ Ulk2 -/- and the MEFs that were isolated and cultured were immortalised SIM using a standard serial passaging protocol.
- Cellosaurus ID: CVCL_5A54
- For Research Use Only
Target Details
- Target: ULK1, ULK2
Application Details
Handling
- Format: Frozen
- Growth medium: DMEM + 20% FCS + 2mM Glutamine + pen/strep
- Shipping conditions: Dry ice
Documentation
Related Tools
References
- • McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.
- • Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.
.svg)
Unit size: 
.svg)
.svg)
