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Contributor Information

  • Name Marie McIlroy
  • Institute Royal College of Surgeons Ireland (RCSI)

Tool Details

  • Tool name: MCF7aro Let-R Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: MCF7
  • Organism: Human
  • Tissue: Breast
  • Cancer type: Breast cancer
  • Disease: Cancer
  • Conditional: Yes
  • Description: MCF7aro Let-R Cell Line is a human cell line model of aromatase inhibitor resistant breast cancer.
  • Research area: Cancer ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: They are cultured in phenol red free Eagle's Minimum Essential Medium (Phenol-red free MEM) (Sigma Aldrich), 10% charcoal dextran stripped FCS (CDS-FCS), 1% Pen-Strep (Sigma Aldrich), 2 mM L-Glutamine (Sigma Aldrich) and 200 µg/ml G418 disulfate salt (Geneticin) (Sigma Aldrich), 2.5-8 M androstenedione and 10-6 M letrozole.
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   Cellosaurus MCF-7aro Let-R (CVCL_W348)
  •   O'Hara et al. 2012. Clin Cancer Res. 18(12):3305-15. PMID: 22550166.
  •   AIB1:ERa transcriptional activity is selectively enhanced in aromatase inhibitor-resistant breast cancer cells.
  •   McIlroy et al. 2010. Cancer Res. 70(4):1585-94. PMID: 20145129.
  •   Interaction of developmental transcription factor HOXC11 with steroid receptor coactivator SRC-1 mediates resistance to endocrine therapy in breast cancer [corrected].