2fTGH-U4A Cell Line

The 2fTGH-U4A Cell Line is part of a panel of IFNy pathway mutant fibrosarcoma cell lines isolated by chemical mutagenesis of IFNy insensitive reporter cells derived from HT1080 cells. Knockout…

Contributors

Inventor Institute
George Stark Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Cat. #: 151809
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Organism: Human
Gender: Male
Model: Mutant
Product description: The 2fTGH-U4A Cell Line is part of a panel of IFNy pathway mutant fibrosarcoma cell lines isolated by chemical mutagenesis of IFNy insensitive reporter cells derived from HT1080 cells. Knockout genes have been identified and span multiple members of the IFMy pathway. These cell lines are be useful for the in vitro study and comparison of disrupted interferon signalling at multiple points across the IFN pathway. The following cell lines are part of the group of IFN signalling mutants: U4C, U2A, U3A, 2FTGH, U6A, U5A. Each containing a different mutation in the IFN signalling pathway.
Gender: Male
CRISPR: Yes
Conditional: Yes
Production details: Human; HT1080 human sarcoma cell lines transfected with a vector encoding a selectable marker regulated by interferon to create the 2fTGH cell line, enabling selection of mutations in genes encoding components of the interferon signalling pathway. Chemical mutagenesis of the 2fTGH cell line enabled isolation of 10 IFNg signalling mutants.
Cellosaurus ID: CVCL_9470
Parental cell line: HT 1080
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-4×10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.
Recommended controls: The wild type 2FTGH human fibrosarcoma as a positive control together with the U5a and U3a IFNB resistant cell lines.
Mycoplasma free: Yes
References:

Haan et al. 2008. J Immunol. 180(2):998-1007. PMID: 18178840.

Dual role of the Jak1 FERM and kinase domains in cytokine receptor binding and in stimulation-dependent Jak activation.

Sun et al. 2004. J Interferon Cytokine Res. 24(6):350-61. PMID: 15212709.

Ectopic expression of toll-like receptor-3 (TLR-3) overcomes the double-stranded RNA (dsRNA) signaling defects of P2.1 cells.

Guo et al. 2000. Virology. 267(2):209-19. PMID: 10662616.

Induction of the human protein P56 by interfero…

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