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Contributor Information

  • Name George Stark
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: 2fTGH-U4A Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: HT 1080
  • Organism: Human
  • Cancer type: Sarcoma
  • Disease: Cancer
  • Model: Mutant
  • Conditional: Yes
  • Description: The 2fTGH-U4A Cell Line is part of a panel of IFNy pathway mutant fibrosarcoma cell lines isolated by chemical mutagenesis of IFNy insensitive reporter cells derived from HT1080 cells. Knockout genes have been identified and span multiple members of the IFMy pathway. These cell lines are be useful for the in vitro study and comparison of disrupted interferon signalling at multiple points across the IFN pathway. The following cell lines are part of the group of IFN signalling mutants: U4C, U2A, U3A, 2FTGH, U6A, U5A. Each containing a different mutation in the IFN signalling pathway.
  • Research area: Cancer; Cell signaling and signal transduction; Immunology
  • Production details: Human; HT1080 human sarcoma cell lines transfected with a vector encoding a selectable marker regulated by interferon to create the 2fTGH cell line, enabling selection of mutations in genes encoding components of the interferon signalling pathway. Chemical mutagenesis of the 2fTGH cell line enabled isolation of 10 IFNg signalling mutants.
  • Cellosaurus ID: CVCL_9470

  • For Research Use Only

Target Details

  • Target: IFN signalling mutant U4A

Application Details


  • Format: Frozen
  • Growth medium: Parental 2fTGH and mutant cell lines can be grown in DMEM with 10% FCS.
  • Shipping conditions: Dry ice



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  •   Mutant U5A cells are complemented by an interferon-alpha beta receptor subunit generated by alternative processing of a new member of a cytokine receptor gene cluster.
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  •   High-frequency mutagenesis of human cells and characterization of a mutant unresponsive to both alpha and gamma interferons.
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  •   Use of a selectable marker regulated by alpha interferon to obtain mutations in the signaling pathway.