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Contributor Information

  • Name Sharon Tooze
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: HEK293-A mRFP-ATG9 Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: HEK293-A
  • Organism: Human
  • Tissue: Embryo
  • Conditional: Yes
  • Description: The HEK293-A mRFP-ATG9 cell line is a stable cell line expressing mRFP-tagged ATG9 (human) in background of HEK293A cell line. It can be used to monitor delivery of membrane from the endosomes and Golgi to the forming autophagosome using live-cell microscopy and biochemical approaches.
  • Research area: Cancer ; Apoptosis and Programmed Cell Death ; Cell Signaling & Signal Transduction ; Cellular Transport ; Drug Discovery & Development ; Metabolism

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: DMEM + 10% FCS + 2mM Glutamine + 400ug/ml geneticin (G418) for maintainance.
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   Orsi et al. 2012. Mol Biol Cell. 23(10):1860-73. PMID: 22456507.
  •   Dynamic and transient interactions of Atg9 with autophagosomes, but not membrane integration, are required for autophagy.
  •   Young et al. 2006. J Cell Sci. 119(Pt 18):3888-900. PMID: 16940348.
  •   Starvation and ULK1-dependent cycling of mammalian Atg9 between the TGN and endosomes.