CELL LINES
Contributor Information
- Name Tomas Lindahl
- Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
- Tool name: MEF OGG1 KO Cell Line
- Tool type: Cell Lines
- Organism: Mouse
- Tissue: Embryo
- Model: Knock-Out
- Description: The MEF OGG1 KO cell line can be used to assess the role of the mammalian enzyme OGG1 in repair of DNA damage and prevention of carcinogenesis. the cell line can be used to assess the incidence and consequences of 8-oxoG formation in the mammalian genome.
- Research area: Cancer; DNA Damage and Repair; Drug Discovery & Development
- Cellosaurus ID: CVCL_6E74
- For Research Use Only
Target Details
- Target: OGG1
Application Details
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Application notes: Primary mouse embryo fibroblast (MEF) cultures from Ogg1 mutant mice were established by standard procedures from individual embryonic day 13.5 (E13.5) embryos derived from heterozygous matings. Cells were cultured in DMEM/Ham
Handling
- Format: Frozen
- Growth medium: DMEM/Ham's F-12 (3:1) with 10% FBS
- Shipping conditions: Dry ice
Documentation
- Available on request
Related Tools
References
- • Klungland et al. 1999. Proc Natl Acad Sci U S A. 96(23):13300-5. PMID: 10557315.
- • Accumulation of premutagenic DNA lesions in mice defective in removal of oxidative base damage.
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