- Name Tomas Lindahl
- Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories
- Tool name: MEF OGG1 KO Cell Line
- Tool type: Cell Lines
- Organism: Mouse
- Tissue: Embryo
- Model: Knock-Out
- Description: The MEF OGG1 KO cell line can be used to assess the role of the mammalian enzyme OGG1 in repair of DNA damage and prevention of carcinogenesis. the cell line can be used to assess the incidence and consequences of 8-oxoG formation in the mammalian genome.
- Research area: Cancer; DNA Damage and Repair; Drug Discovery & Development
- Cellosaurus ID: CVCL_6E74
- For Research Use Only
- Target: OGG1
- Application notes: Primary mouse embryo fibroblast (MEF) cultures from Ogg1 mutant mice were established by standard procedures from individual embryonic day 13.5 (E13.5) embryos derived from heterozygous matings. Cells were cultured in DMEM/Hams F-12 (3:1) with 10% FBS and genotyped by Southern hybridization, and permanent cell lines were established from transformed clones arising spontaneously after repeated passage in culture.
- Format: Frozen
- Growth medium: DMEM/Ham's F-12 (3:1) with 10% FBS
- Shipping conditions: Dry ice
- • Klungland et al. 1999. Proc Natl Acad Sci U S A. 96(23):13300-5. PMID: 10557315.
- • Accumulation of premutagenic DNA lesions in mice defective in removal of oxidative base damage.