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Contributor Information

  • Name Joyce Taylor-Papadimitriou ; Joy Burchell
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: E3 STn Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: E3
  • Organism: Mouse
  • Tissue: Breast
  • Cancer type: Carcinoma
  • Disease: Cancer
  • Model: Knock-In
  • Conditional: Yes
  • Description: The E3 STn Cell Line is a mouse mammary carcinoma cell line E3 which expresses human MUC1 and Sialyl-Tn. Changes in the composition of glycans added to glycoproteins and glycolipids are characteristic of the change to malignancy. Sialyl-Tn (STn) is expressed by 2530% of breast carcinomas but its expression on normal tissue is highly restricted. Sialyl-Tn is an O-linked disaccharide that can be carried on various glycoproteins. One such glycoprotein MUC1 is expressed by the vast majority of breast carcinomas. Both STn and MUC1 have been considered as targets for immunotherapy of breast cancer patients.
  • Research area: Cancer; Drug development; Immunology
  • Production details: E3 cell line stably transfected with murine ST6GalNAc I leading to expression of the MUC1 STn epitope. The E3 cell lines were developed from the 410.4 cell line by transfection of the hygromycin resistance gene with the MUC1 gene. Calcium phosphate transfection of E3 cells was performed using the pbabe-neo gene with or without the cDNA encoding murine B7.1. Stable transfection of murine ST6GalNAc I was performed to obtain the cell line E3-STn.
  • Cellosaurus ID: CVCL_W346
  • Additional notes: E3-hMUC1 stably transfected with murine ST6GalNAc I leading to expression of both MUC1 and STn

  • For Research Use Only

Target Details

  • Target: mST6-Gal-NAc and MUC1

Application Details

  • Application notes: E3-hMUC1 stably transfected with murine ST6GalNAc I leading to expression of both MUC1 and STn

Handling

  • Format: Frozen
  • Growth medium: Dulbecco's E4 medium containing 600 ?„?žg/ml G418, 200 ?„?žg/ml hygromycin and 10% fetal calf serum (FCS)
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

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References

  • •  Julien et al. 2009. Br J Cancer. 100(11):1746-54. PMID: 19436292.
  • •  Sialyl-Tn vaccine induces antibody-mediated tumour protection in a relevant murine model.