T47D-182R2 Cell Line
T47D-182R2 is an adherent human breast cancer cell line that exhibits resistance to fulvestrant (Faslodex). It was developed from the parental T47D/S5 cell line through long-term exposure to 100 nM fulvestrant.
Contributors
| Inventor | Institute |
|---|---|
| Anne Lykkesfeldt | Danish Cancer Society |
| Cat. #: | 151890 |
|---|---|
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Breast cancer |
| Research Fields: | Cancer;Drug development |
| Organism: | Human |
| Tissue: | Breast |
| Model: | Cancer cell line |
| Growth properties: | Adherent |
| Cancer Types In Detail: | Breast cancer;Fulvestrant resistant |
| Product description: | T47D-182R2 is an adherent human breast cancer cell line that exhibits resistance to fulvestrant (Faslodex). It was developed from the parental T47D/S5 cell line through long-term exposure to 100 nM fulvestrant. The cells retain an epithelial morphology. As resistance to fulvestrant—a common second-line endocrine therapy—is a clinical challenge, T47D-182R2 represents a valuable model for studying acquired resistance mechanisms. It serves as a robust tool for identifying targeted therapies against resistant tumor cells, as well as for evaluating strategies to prevent or delay resistance emergence. |
|---|---|
| Production details: | Human breast cancer cell line derived from T47D/S5 by long term treatment with 100 nM fulvestrant. |
| Cellosaurus ID: | CVCL_1D35 |
| Parental cell line: | T47D/S5 |
| disease: | Cancer |
| Format: | Frozen |
|---|---|
| Shipping conditions: | Dry ice |
| Growth medium: | Phenol red free RPMI 1640 + 5% FCS + glutamax + 8ug Insulin/ml + 100 nM fulvestrant. Fetal Calf Serum (FCS) typically contains less estrogen than Fetal Bovine Serum (FBS) and is the preferred supplement for this cell line. |
| Subculture routine: | Following thawing and counting, dilute the cell suspension with sufficient medium and distribute 5 mL each into T25 flasks to achieve a seeding density of 1.8 – 2.0 x 10^4 / cm2. Place in 37°C, 5% CO₂ incubator. Change medium after 24 hours to remove residual DMSO and then every 2-3 days. Subculture routine: Split 1:7 weekly (slow growing cell line) with Trypsin-EDTA for detachment at 37 °C for 5 minutes. Please also see detailed protocol within the Product Datasheet in the Documentation section below. |
| Temperature: | 37°C |
| Atmosphere: | 5% CO₂ |
| Storage medium: | 10% DMSO in FCS |
| References: |
Larsen et al. 2015. PLoS One. 10(2):e0118346. PMID: 25706943. Larsen et al. 2015. BMC Cancer. 15:239. PMID: 25885472. Aurora kinase B is important for antiestrogen resistant cell growth and a potential biomarker for tamoxifen resistant breast cancer. SRC drives growth of antiestrogen resistant breast cancer cell lines and is a marker for reduced benefit of tamoxifen treatment. Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268. T47D breast cancer cells switch from ER… |
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