MCF7/TAMR-8 Cell Line
The MCF7/TAMR-8 cell line is a good model cell to study the signalling pathways, which are the major drivers of tamoxifen-resistant growth.
Contributors
| Inventor | Institute |
|---|---|
| Anne Lykkesfeldt | Danish Cancer Society |
| Cat. #: | 152086 |
|---|---|
| Tool sub type: | Continuous |
| Unit size: | 1×10^6 cells / vial |
| Cancer types: | Breast cancer;Breast cancer |
| Organism: | Human |
| Tissue: | Breast |
| Gender: | Female |
| Model: | Tumour line |
| Growth properties: | Adherent |
| Alternate name: | MCF-7/TAMR-8; TAMR-8 |
|---|---|
| Product description: | MCF7/TAMR-8 cell line is a stable tamoxifen-resistant subline. This cell line has been established in tissue culture after long term treatment with 1 uM tamoxifen. Tamoxifen (Nolvadex) is a widely used drug for hormone-dependent cancer. Tamoxifen resistance (either primary or acquired) makes oestrogen receptor-positive breast cancer much more difficult to treat. This cell line was produced from the parental cell line MCF7/S0.5, as a model cell system to study the effects of tamoxifen resistant cancer growth. Tamoxifen-resistant cells are passaged continuously in the presence of 1 uM tamoxifen. This concentration is lethal for the parental cell line. MCF7/TAMR-8 cells are oestrogen receptor positive and progesterone receptor negative. Previous applications of this cell line include treatment with steroidal antioestrogens. MCF7/TAMR-8 enables identification of new hormone therapies and greater understanding of the signalling pathways/methods behind tamoxifen resistance. Additionally MCF/TAMR-8 can aid in identifying new predictive markers for response to hormonal therapy. |
| Gender: | Female |
| CRISPR: | Yes |
| Conditional: | Yes |
| Production details: | The parental cell line for the MCF7/TAMR-8 cells is MCF7/S0.5, which was adapted to grow at low serum concentration in order to study the effect of estradiol and tamoxifen. MCF7/TAMR-8 has been established from a clone of cells that survived long term treatment with 1 uM tamoxifen. Tamoxifen-resistant cells are passaged continuously in presence of 1 uM tamoxifen, which is lethal for the parental MCF7/S0.5 cell line. |
| Additional notes: | MCF7/TAMR-8 cells are oestrogen receptor positive and progesterone receptor negative. MCF7/TAMR-8 cells are growth inhibited by the pure antioestrogen fulvestrant. The oestrogen receptor is a major driver of growth of MCF7/TAMR-8 cell. |
| Cellosaurus ID: | CVCL_1D44 |
| Parental cell line: | MCF7 S0.5 |
| disease: | Cancer;Cancer |
| Format: | Frozen |
|---|---|
| Storage conditions: | Liquid Nitrogen |
| Shipping conditions: | Dry ice |
| Growth medium: | Phenol red-free DMEM:Ham's F-12 containing 1% fetal bovine serum, 2 mM Glutamax and 6 ng/ml insulin. To maintain high-level resistance, medium was supplemented with Tamoxifen (1 uM). |
| Temperature: | 37° C |
| Atmosphere: | 5% CO2 |
| Recommended controls: | MCF7-S0.5 parental line |
| Mycoplasma free: | Yes |
| Biosafety level: | 1 |
| References: |
Thrane et al. 2015. Oncogene. 34(32):4199-210. PMID: 25362855. Elias et al. 2015. Oncogene. 34(15):1919-1927. PMID: 24882577. Pedersen et al. 2014. Int J Oncol. 45(5):2167-2175. PMID: 25175082. Lundqvist et al. 2014. Steroids. 85:30-35. PMID: 24747771. Thrane et al. 2013. Breast Cancer Res Treat. 139(1):71-80. PMID: 23609470. Larsen et al. 2012. Int J Oncol. 41(5):1863-1870. PMID: 22961366. Cutrupi et al. 2012. Oncogene. 31(40):4353-4361. PMID: 22249258. Frankel et al. 2007. Breast Cancer Res Treat. 104(2):165-179. PMID: 17061041. Beliakoff et al. 2003. Clin Cancer Res. 9(13):4961-4971. PMID: 14581371. Madsen et al. 1995. Mol Cell Endocrinol. 109(2):197-207. PMID: 7664983. |
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