MCF7/TAMR-8 Cell Line
- Name Anne Lykkesfeldt
- Institute Danish Cancer Society
Tool name: MCF7/TAMR-8 Cell Line
Tool type: Cell Lines
Tool sub-type: Continuous
Parental cell line: MCF7 S0.5
Cancer type: Breast cancer, tamoxifen-resistant
Description: The MCF7/TAMR-8 Cell line is a breast cancer cell line resistant to tamoxifen. Tamoxifen is by far the most widely used drug for hormone-dependent breast cancer. However, primary or acquired resistance to Tamoxifen severely limits its clinical effectiveness. The MCF7/TAMR-8 cell line is a good model cell to study the signalling pathways, which are the major drivers of tamoxifen-resistant growth.
The TAMR lines were established from the MCF7/S0.5 cell line, which was adapted to grow with 0.5% fetal calf serum in phenol red containing DMEM/F12 medium. Treatment with tamoxifen was started in passage 351. Few colonies of cells survived the treatment and after 28 days of tamoxifen treatment, tamoxifen was omitted from the medium for 22 days. After 19 passages without tamoxifen (passage 372) the cells underwent a second treatment with tamoxifen which initially reduced cell growth rate, but around 390-400 the growth rate of the tamoxifen resistant cell lines was close to the growth rate of the parental MCF7/S0.5 cells.
Research area: Cancer ; Drug Discovery & Development
- For Research Use Only
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