CELL LINES
Contributor Information
- Name Anne Lykkesfeldt
- Institute Danish Cancer Society
Tool Details
- Tool name: MCF7/164R-1 Cell Line
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: MCF7 S0.5
- Organism: Human
- Tissue: Breast
- Cancer type: Breast; Fulvestrant resistant
- Disease: Cancer
- Model: Tumour line
- Description: The MCF7/164R-1 Cell line is a breast cancer cell line resistant to fulvestrant. Treatment with the steroidal antiestrogen fulvestrant has proven effective upon progression on tamoxifen therapy and is now approved for second-line treatment after tamoxifen or aromatase inhibitors. As for tamoxifen treatment of advanced breast cancer, resistance will inevitably occur also for fulvestrant. Clarification of the molecular changes associated with the resistant growth is needed to find targeted treatments to resistant tumour cells and treatments that can inhibit or delay the emergence of resistance.
- Research area: Cancer; Drug Discovery & Development
- Production details: The MCF7/164R-1 cell line has been established from a clone of MCF7/S0.5 cells surviving long term growth with the pure steroidal antiestrogen ICI 164,384 in 100 nM concentration, see Lykkesfeldt et al 1995. The MCF7/164R-1 cells are also resistant to the pure steroidal antiestrogen fulvestrant (ICI 182,780) and can be maintained continuously in growth medium with 100 nM fulvestrant.
- Cellosaurus ID: CVCL_1D48
- Additional notes: Upon withdrawal of fulvestrant, the cells express ER alpha, although at a reduced level compared to the parental MCF7/S0.5 cell line. The MCF7/164R-1 cells do not express progesterone receptor. The MCF7/164R-1 cells express increased level of EGFR, phosphorylated EGFR and phosphorylated ErbB3 and reduced level of ErbB4 compared to the parental MCF7/S0.5 cells.
- For Research Use Only
Target Details
- Target: Oestrogen receptor
Application Details
- Application notes: Upon withdrawal of fulvestrant, the cells express ER alpha, although at a reduced level compared to the parental MCF7/S0.5 cell line. The MCF7/164R-1 cells do not express progesterone receptor. The MCF7/164R-1 cells express increased level of EGFR, phosphorylated EGFR and phosphorylated ErbB3 and reduced level of ErbB4 compared to the parental MCF7/S0.5 cells. Passage 423 (AL3802, AL3803)
Handling
- Format: Frozen
- Passage number: Passage 403 (AL3810, AL3811)
- Growth medium: Phenol red free DMEM/F12 (1:1) supplemented with 1% FCS, Glutamax 2.5 mM and 6 ng/ml insulin. Supplemented with 100nM fulvestrant to maintain resistance.
- Shipping conditions: Dry ice
Documentation
- Available on request
References
- • Thrane et al. 2015. Oncogene. 34(32):4199-210. PMID: 25362855.
- • A kinase inhibitor screen identifies Mcl-1 and Aurora kinase A as novel treatment targets in antiestrogen-resistant breast cancer cells.
- • Sonne-Hansen et al. 2010. Breast Cancer Res Treat. 121(3):601-13. PMID: 19697122.
- • Breast cancer cells can switch between estrogen receptor alpha and ErbB signaling and combined treatment against both signaling pathways postpones development of resistance.
- • Frogne et al. 2009. Breast Cancer Res Treat. 114(2):263-75. PMID: 18409071.
- • Activation of ErbB3, EGFR and Erk is essential for growth of human breast cancer cell lines with acquired resistance to fulvestrant.
- • Madsen et al. 1997. Cancer Res. 57(4):585-9. PMID: 9044830.
- • Estrogen receptor messenger RNA splice variants are not involved in antiestrogen resistance in sublines of MCF-7 human breast cancer cells.
- • Lykkesfeldt et al. 1995. Int J Cancer. 61(4):529-34. PMID: 7759159.
- • Human breast cancer cell lines resistant to pure anti-estrogens are sensitive to tamoxifen treatment.
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