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Contributor Information

  • Name Anne Lykkesfeldt
  • Institute Danish Cancer Society

Tool Details

  • Tool name: MCF7/182R-1 Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: MCF7 S0.5
  • Organism: Human
  • Tissue: Breast
  • Cancer type: Breast cancer, fulvestrant resistant
  • Disease: Cancer
  • Description: The MCF7/182R-1 Cell line is a breast cancer cell line resistant to fulvestrant. Treatment with the steroidal antiestrogen fulvestrant has proven effective upon progression on tamoxifen therapy and is now approved for second-line treatment after tamoxifen or aromatase inhibitors. As for tamoxifen treatment of advanced breast cancer, resistance will inevitably occur also for fulvestrant. Clarification of the molecular changes associated with the resistant growth is needed to find targeted treatments to resistant tumour cells and treatments that can inhibit or delay the emergence of resistance.
  • Research area: Cancer ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Passage number: Passage 412 (AL3423, AL3424)
  • Growth medium: Phenol red free DMEM/F12 (1:1) supplemented with 1% FCS, Glutamax 2.5 mM and 6 ng/ml insulin. Supplemented with 100nM fulvestrant to maintain resistance.
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Thrane et al. 2014. Oncogene. PMID: 25362855.
  •   A kinase inhibitor screen identifies Mcl-1 and Aurora kinase A as novel treatment targets in antiestrogen-resistant breast cancer cells.
  •   Frogne et al. 2009. Breast Cancer Res Treat. 114(2):263-75. PMID: 18409071.
  •   Activation of ErbB3, EGFR and Erk is essential for growth of human breast cancer cell lines with acquired resistance to fulvestrant.
  •   Frankel et al. 2007. Breast Cancer Res Treat. 104(2):165-79. PMID: 17061041.
  •   Protein Kinase C alpha is a marker for antiestrogen resistance and is involved in the growth of tamoxifen resistant human breast cancer cells.
  •   Frogne et al. 2005. Endocr Relat Cancer. 12(3):599-614. PMID: 16172194.
  •   Antiestrogen-resistant human breast cancer cells require activated protein kinase B/Akt for growth.
  •   Bradshaw et al. 2004. Curr Med Chem. 11(8):1009-21. PMID: 15078163.
  •   The development of the antitumour benzothiazole prodrug, Phortress, as a clinical candidate.
  •   Lykkesfeldt et al. 1995. Int J Cancer. 61(4):529-34. PMID: 7759159.
  •   Human breast cancer cell lines resistant to pure anti-estrogens are sensitive to tamoxifen treatment.