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Contributor Information

  • Name Anne Lykkesfeldt
  • Institute Danish Cancer Society

Tool Details

  • Tool name: T47D/TR-1 Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: T47D/S2
  • Organism: Human
  • Tissue: Breast
  • Cancer type: Breast cancer
  • Disease: Cancer
  • Model: Tumour line
  • Conditional: Yes
  • Description: The T47D/TR-1 Cell line is a breast cancer cell line resistant to tamoxifen. Antiestrogen resistance is a major problem in breast cancer treatment. Therefore, the search for new therapeutic targets and biomarkers for antiestrogen resistance is crucial. This cell line allows the study of the mechanisms involved in tamoxifen resistant breast cancer cell growth. T47D/TR-1 cells are ER alpha positive and express progesterone receptor, although at reduced level compared to parental T7D/S2 cells. T47D/TR-1 are growth inhibited by fulvestrant. Passage 164 (AL3569, AL3570)
  • Research area: Cancer; Drug development
  • Production details: The T47D/TR-1 cell line has been established by long term treatment of T47D/S2 cells with 1 Â?„?žM tamoxifen. Clonal selection was performed in medium without tamoxifen. After growth in presence of 1 Â?„?žM tamoxifen for 10 months, the growth rate was similar to the parental cells.
  • Cellosaurus ID: CVCL_1D36

  • For Research Use Only

Target Details

  • Target: Oestrogen receptor

Application Details

  • Application notes: T47D/TR-1 cells are ER alpha positive and express progesterone receptor, although at reduced level compared to parental T7D/S2 cells. T47D/TR-1 are growth inhibited by fulvestrant.

Handling

  • Format: Frozen
  • Passage number: Passage 164 (AL3569, AL3570)
  • Growth medium: Phenol red free RPMI 1640 + 2% FCS + glutamax + 8?„?žg Insulin/ml + 1 uM tamoxifen
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

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References

  • •  Larsen et al. 2015. PLoS One. 10(2):e0118346. PMID: 25706943.
  • •  Larsen et al. 2015. BMC Cancer. 15:239. PMID: 25885472.
  • •  Aurora kinase B is important for antiestrogen resistant cell growth and a potential biomarker for tamoxifen resistant breast cancer.
  • •  SRC drives growth of antiestrogen resistant breast cancer cell lines and is a marker for reduced benefit of tamoxifen treatment.
  • •  Thrane et al. 2014. Oncogene. :. PMID: 25362855.
  • •  A kinase inhibitor screen identifies Mcl-1 and Aurora kinase A as novel treatment targets in antiestrogen-resistant breast cancer cells.
  • •  Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.
  • •  T47D breast cancer cells switch from ER/HER to HER/c-Src signaling upon acquiring resistance to the antiestrogen fulvestrant.