T47D/S2 Cell Line

Tool Details

*FOR RESEARCH USE ONLY

• Name: T47D/S2 Cell Line
• Tool sub type: Continuous
• Parental cell: T47D
• Organism: Human
• Gender: Female
• Tissue: Breast
• Disease: Cancer
• Model: Tumour line
• Conditional: Yes
• Description: The T47D/S2 cell line is a control cell line for the tamoxifen resistant T47D/TR-1 and T47D/TR-2 cell lines. This cell line has been established by the reduction of the serum concentration in order to enable growth inhibiton with tamoxifen. T47D/S2 is adherent and the morphology is polygonal epithelial. T47D/S2 cells express oestrogen receptor (alpha) and progesterone receptor. This cell line allows the study of the mechanisms involved in tamoxifen resistant breast cancer cell growth.
• Application: Determining molecular mechanisms around tamoxifen resistance
• Production details: T47D/S2 cells have been established by reduction of the serum concentration to 2% fetal calf serum, in order to enable growth inhibition with tamoxifen.
• Biosafety level: 1
• Cellosaurus id: CVCL_1D45

Target Details

• Target: Oestrogen receptor

Applications

• Application: Determining molecular mechanisms around tamoxifen resistance
• Application notes: T47DS2 cells express ER alpha and progesterone receptor.

Handling

• Format: Frozen
• Passage number: Passage 151 (AL3707, AL3708)
• Growth medium: Phenol red free RPMI 1640 + 2% FCS + glutamax + 8ug Insulin/ml
• Temperature: 37° C
• Atmosphere: 5% CO2
• Unit size: 1x10^6 cells / vial
• Shipping conditions: Dry ice
• Storage conditions: Liquid Nitrogen
• Mycoplasma free: Yes

Related Tools

• Related tools: T47D/TR-1 Cell Line ; T47D/TR-2 Cell Line ; T47D-182R2 Cell Line ; T47D-182R1 Cell Line

References

• Larsen et al. 2015. PLoS One. 10(2):e0118346. PMID: 25706943.
• Larsen et al. 2015. BMC Cancer. 15(1):1-15. PMID: 25885472.
• Thrane et al. 2014. Oncogene. 34(32):4199-4210. PMID: 25362855.
• Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.