Product Image

Contributor Information

  • Name Anne Lykkesfeldt
  • Institute Danish Cancer Society

Tool Details

  • Tool name: MCF7/LetR-3 Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: MCF7
  • Organism: Human
  • Tissue: Breast
  • Cancer type: Breast Cancer
  • Disease: Cancer
  • Description: The MCF7/LetR-3 Cell Line was developed as a model of resistance to anti-cancer treatment with aromatase inhibitors. Third generation aromatase inhibitors (AIs) have proven to be effective treatment for estrogen receptor positive (ER+) breast cancer and are today recommended as first line endocrine therapy for postmenopausal ER+ breast cancer patients, making up the majority of breast cancer patients. However, a major problem is development of resistance against AIs. Since molecular mechanisms of AI resistance are largely undisclosed, the development of cell lines resistant to the non-steroidal AI letrozole allows the study of the molecular basis for resistance to AIs to unravel new targets for treatment.
  • Research area: Cancer ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Passage number: Passage 436 (AL3136, AL3137)
  • Growth medium: Phenol-red-free DMEM/F12 medium supplemented with 10% newborn calf serum, 2.5 mM Glutamax, 6 ng/ ml insulin, 0.1 uM testosterone and 1 uM letrozole.
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Hole et al. 2015. Breast Cancer Res Treat. 149(3):715-26. PMID: 25667100.
  •   Hole et al. 2015. Int J Oncol. 46(4):1481-90. PMID: 25625755.
  •   Aurora kinase A and B as new treatment targets in aromatase inhibitor-resistant breast cancer cells.
  •   New cell culture model for aromatase inhibitor-resistant breast cancer shows sensitivity to fulvestrant treatment and cross-resistance between letrozole and exemestane.