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Contributor Information

  • Name Jennifer Nichols
  • Institute University of Oxford

Tool Details

  • Tool name: LF2 Cell Line
  • Tool type: Cell Lines
  • Organism: Mouse
  • Tissue: Embryo
  • Conditional: No
  • Description: The LF2 mouse embryonic stem cell line is a germ-line competent embryonic stem cell line that inactivates one of its X chromosomes upon retinoic acid treatment. Embryonic stem cells provide a powerful system for the experimental analysis of mammalian development. Their genetic manipulation allows the introduction of defined modifications into the mouse germ-line, whilst in culture they constitute both an assay system for, and a potential source of, embryonic regulatory factors.
  • Research area: Genetic Studies Tools ; Stem Cell Biology
  • Cellosaurus ID: CVCL_AS05

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Embryonic bodies were formed by growing cells in low adherence dishes in DMEM+FBS for 10 days.
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Evans et al. Development 110, 1341-1348 (1990).