Cat. #152596
SVCT-MI2 Cell Line
Cat. #: 152596
Unit size: 1x10^6 cells / vial
Organism: Human
Tissue: Breast
Disease: Cancer
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Joyce Taylor-Papadimitriou ; Sidney Chang
Institute: Cancer Research UK, Lincoln's Inn Fields Institute
Primary Citation: Yamashita et al. 2009. Bioorg Med Chem. 17(17):6286-6291. PMID: 19674905.
Tool Details
*FOR RESEARCH USE ONLY
- Name: SVCT-MI2 Cell Line
- Organism: Human
- Gender: Female
- Tissue: Breast
- Disease: Cancer
- Growth properties: Adherent
- Conditional: No
- Description: SVCT-MI2 has been established from human breast epithelial cells being transfected with simian virus 40 (SV40) - a known oncogenic DNA virus in addition to a plasmid containing the neomycin gene and the human oncogene EJ Harvey-ras. This transformed epithelial cell line shows tumourigenicity in nude mice (post-selection of G418-resistant colonies). SVCT-MI2 enables molecular analysis of SV40 in human breast cancers.
- Application: Molecular analysis of SV40 in breast cancer
- Cellosaurus id: CVCL_2719
Target Details
- Target: Human oncogene EJ Harvey-ras
Applications
- Application: Molecular analysis of SV40 in breast cancer
Handling
- Format: Frozen
- Growth medium: RPMI 1640 or DMEM + 2mM Glutamine + 10ug / ml insulin + 5ug / ml hydrocortisone + 10% FBS. Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA. Note: cells may pile up when confluent.
- Temperature: 37° C
- Atmosphere: 5% CO2
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
Related Tools
- Related tools: SVCT Cell Line
References
- Yamashita et al. 2009. Bioorg Med Chem. 17(17):6286-6291. PMID: 19674905.
