Product Image

Contributor Information

  • Name Joyce Taylor-Papadimitriou ; Sidney Chang
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: SVCT-MI2 Cell Line
  • Tool type: Cell Lines
  • Parental cell line:
  • Organism: Human
  • Tissue: Breast
  • Cancer type: Breast Cancer
  • Disease: Cancer
  • Description: SVCT-MI2 was established in 1987 from epithelial cells in breast milk secretions. The cells have been transfected by microinjection using SV40 DNA and a plasmid containing the neomycin gene and the human oncogene EJ Harvey-ras. After selection of G418-resistant colonies, the clone SVCT-MI2 was shown to be tumorigenic in nude mice. They also have the ability to grow anchorage-independent if cultured in soft agar.
  • Research area: Cancer ; Cell Cycle ; Drug Discovery & Development ; Epigenetics & Nuclear Signalling

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: RPMI 1640 or DMEM + 2mM Glutamine + 10µg / ml insulin + 5µg / ml hydrocortisone + 10% FBS. Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Note: cells may pile up when confluent.
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Yamashita et al. 2009. Bioorg Med Chem. 17(17):6286-91. PMID: 19674905.
  •   Synthesis and evaluation of bioactive naphthoquinones from the Brazilian medicinal plant, Tabebuia avellanedae.