CELL LINES
Contributor Information
- Name Joyce Taylor-Papadimitriou ; Sidney Chang
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: SVCT-MI2 Cell Line
- Tool type: Cell Lines
- Organism: Human
- Tissue: Breast
- Cancer type: Breast
- Disease: Cancer
- Growth properties: Adherent
- Conditional: No
- Description: SVCT-MI2 was established in 1987 from epithelial cells in breast milk secretions. The cells have been transfected by microinjection using SV40 DNA and a plasmid containing the neomycin gene and the human oncogene EJ Harvey-ras. After selection of G418-resistant colonies, the clone SVCT-MI2 was shown to be tumorigenic in nude mice. They also have the ability to grow anchorage-independent if cultured in soft agar.
- Research area: Cancer; Cell Cycle; Drug Discovery & Development; Epigenetics & Nuclear Signalling
- Cellosaurus ID: CVCL_2719
- For Research Use Only
Target Details
- Target: Human oncogene EJ Harvey-ras
Application Details
Handling
- Format: Frozen
- Growth medium: RPMI 1640 or DMEM + 2mM Glutamine + 10Ğg / ml insulin + 5Ğg / ml hydrocortisone + 10% FBS. Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Note: cells may pile up when confluent.
- Shipping conditions: Dry ice
Documentation
- Available on request
Related Tools
References
- • Yamashita et al. 2009. Bioorg Med Chem. 17(17):6286-91. PMID: 19674905.
- • Synthesis and evaluation of bioactive naphthoquinones from the Brazilian medicinal plant, Tabebuia avellanedae.
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