- Name Joyce Taylor-Papadimitriou ; Joy Burchell
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
- Tool name: E3 Cell Line
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: 410.4
- Organism: Mouse
- Tissue: Breast
- Cancer type: Mammary
- Disease: Cancer; Immunological diseases
- Model: Immortalised Line
- Description: The E3 cell line was developed to examine the possibility of using the MUC1 gene and its products in active immunization against breast and other carcinomas. The human MUC1 gene codes for a type I membrane glycoprotein that is normally expressed on the apical surface of most glandular epithelial cells, but which is upregulated and under- or differently glycosylated in carcinomas; these differences in glycosylation lead to the exposure of novel epitopes which are not found on the normally processed mucin and can therefore be used as targets.
- Research area: Cancer; Drug Discovery & Development; Immunology
- Production details: The E3 cell line was developed from the 410.4 murine mammary cell line by transfection of the hygromycin resistance gene with the MUC1 gene.
- Cellosaurus ID: CVCL_W345
- For Research Use Only
- • Julien et al. 2009. Br J Cancer. 100(11):1746-54. PMID: 19436292.
- • Sialyl-Tn vaccine induces antibody-mediated tumour protection in a relevant murine model.
- • Smith et al. 1999. Immunology. 97(4):648-55. PMID: 10457219.
- • Expression of B7.1 in a MUC1-expressing mouse mammary epithelial tumour cell line inhibits tumorigenicity but does not induce autoimmunity in MUC1 transgenic mice.
- • Lalani et al. 1991. J Biol Chem. 266(23):15420-6. PMID: 1714457.
- • Expression of the gene coding for a human mucin in mouse mammary tumor cells can affect their tumorigenicity.