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Contributor Information

  • Name Anne Lykkesfeldt
  • Institute Danish Cancer Society

Tool Details

  • Tool name: MCF7/S9 Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: MCF7
  • Organism: Human
  • Tissue: Breast
  • Cancer type: Breast cancer
  • Disease: Cancer
  • Growth properties: Grow in serum free medium. Estrogen and progesterone receptor positive. Estrogen and antiestrogen responsive.
  • Conditional: No
  • Description: The MCF7/S9 cell line was established to grow in serum free medium to enable studies of the effect of estrogens and antiestrogens under conditions without influence of estrogenic compounds supplied via the serum content in the growth medium. We did not find growth stimulation with estradiol, but after the discovery that phenol red had estrogenic activity, the MCF7/S9 cell line was transferred to phenol red free medium, and in this medium. we observed estrogen stimulation of cell growth.
  • Research area: Cancer; Drug development
  • Production details: MCF7/S9 has been established by gradually adapting MCF7 cells to grow at a low serum concentration before transfer to serum-free medium. Since passage no. 387, the cell line has been propagated in phenol red-free DMEM/F12 (1:1) without addition of serum or any growth factor (standard medium for MCF7/S9), in T-flasks coated with collagen. The MCF7/S9 was subcultured once a week with a split ratio of approximately 1:10 and growth medium was changed every second or third day.
  • Cellosaurus ID: CVCL_AS09

  • For Research Use Only

Target Details

Application Details


  • Format: Frozen
  • Growth medium: Phenol-red-free DMEM/F12 medium supplemented with 2.0 mM Glutamax, 10 ug/ml Transferrin, 0.1 mg/ml bovine serum albumin, culture flasks were coated with collagen IV.
  • Shipping conditions: Dry ice



  •   Jensen et al. 2003. J Steroid Biochem Mol Biol. 84(4):469-78. PMID: 12732292.
  •   Effect of antiestrogens and aromatase inhibitor on basal growth of the human breast cancer cell line MCF-7 in serum-free medium.
  •   Briand et al. 1986. Anticancer Res. 6(1):85-90. PMID: 3513694.
  •   Long-term cultivation of a human breast cancer cell line, MCF-7, in a chemically defined medium. Effect of estradiol.