Product Image

Contributor Information

  • Name Sharon Tooze
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: MEF ULK1/2 WT(SV40) Cell Line
  • Tool type: Cell Lines
  • Organism: Mouse
  • Tissue: Embryo
  • Disease: Cancer; Neurodegeneration
  • Growth properties: Autophagy
  • Conditional: Yes
  • Description: The MEF ULK1/2 WT cell line can be used as a wild-type control with ULK1 and ULK2 cell lines and is useful for studying ULK1-and ULK-2 dependent processes, including autophagy. Mouse embryonic fibroblasts derived from a wild-type littermate to the ULK1 KO and immortalized with SV40 large T-antigen.
  • Research area: Cancer; Apoptosis and autophagy; Cell signaling and signal transduction; Metabolism; Neurobiology
  • Production details: Cells were derived from mouse embryos at E13.5. Embryos were decapitated and eviscerated and the remaining tissues were dissociated to obtain MEFs. This cell line is a primary cell line immortalized by SV40.
  • Cellosaurus ID: CVCL_5A51

  • For Research Use Only

Target Details

  • Target: ULK1

Application Details

Handling

  • Format: Frozen
  • Growth medium: DMEM + 20% FCS + 2mM Glutamine + pen/strep
  • Shipping conditions: Dry ice

Documentation

Related Tools

Product Image

ZR-75-1 [XIV-6] cell line

#154618
Product Image

MCF10A-EGFR1m-6 cell line

#161173
Product Image

MCF7A BCAR3 [MCF7-BCAR3] cell line

#154637

References

  • •  McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.
  • •  Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.
  • •  Chan et al. 2009. Mol Cell Biol. 29(1):157-71. PMID: 18936157.
  • •  Kinase-inactivated ULK proteins inhibit autophagy via their conserved C-terminal domains using an Atg13-independent mechanism.