Cat. #152610
MEF ULK2 KO (SV40) Cell Line
Cat. #: 152610
Unit size: 1x10^6 cells / vial
Organism: Mouse
Tissue: Embryo
Disease: Cancer
Model: Knock-Out
£575.00
This fee is applicable only for non-profit organisations. If you are a for-profit organisation or a researcher working on commercially-sponsored academic research, you will need to contact our licensing team for a commercial use license.
Contributor
Inventor: Sharon Tooze
Institute: Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
*FOR RESEARCH USE ONLY
- Name: MEF ULK2 KO (SV40) Cell Line
- Organism: Mouse
- Tissue: Embryo
- Disease: Cancer
- Growth properties: Fibroblast
- Model: Knock-Out
- Description: The MEF ULK2 KO (SV40) cell line provides a tool for the study of Ulk2 and of Autophagy and has a comparable genetic background to MEF Ulk1 Ulk2 DKO and MEF Ulk1 KO cell lines. This cell line is derived from an embryo with a mixed genetic background (Blk6/129B Agouti) and immortalized with SV40 large T-antigen.
- Production details: Primary embryonic fibroblasts were isolated from the embryos of a pregnant female mouse at day 13p.c. The embryos were genotyped to identify those that were Ulk1 +/+ Ulk2 -/- and the MEFs that were isolated and cultured were immortalised SV40 using a standard serial passaging protocol.
- Cellosaurus id: CVCL_5A55
Target Details
- Target: ULK1, ULK2
Handling
- Format: Frozen
- Growth medium: DMEM + 20% FCS + 2mM Glutamine + pen/strep
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
Related Tools
- Related tools: MEF ULK1/2 WT (SIM) Cell Line ; MEF ULK1 KO (SIM) Cell Line ; MEF ULK1/2 WT(SV40) Cell Line ; MEF ULK2 KO (SIM) Cell Line ; MEF ULK1 KO (SV40) Cell Line ; MEF ULK1 ULK2 DKO (SIM) Cell Line ; MEF ULK1 ULK2 DKO (SV40) Cell Line
References
- McAlpine et al. 2013. Autophagy. 9(3):361-73. PMID: 23291478.
- Regulation of nutrient-sensitive autophagy by uncoordinated 51-like kinases 1 and 2.
