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Contributor Information

  • Name Carlos Caldas
  • Institute Cancer Research UK, Cambridge Institute

Tool Details

  • Tool name: HCT 116 BRCA2 -/- [42] Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: HCT 116
  • Organism: Human
  • Tissue: Colon
  • Cancer type: Colon Carcinoma
  • Disease: Cancer
  • Model: Knock-Out
  • Conditional: No
  • Description: BRCA2 -/- HCT116 human colorectal carcinoma cells were generated by homologous recombination to study the role of BRCA2 in DNA repair and to identify additional functions of the BRCA2 gene. These cells show phenotypes consistent with previous reports, including loss of Rad51 foci in the presence of double-strand breaks, chromosomal rearrangements and elevated sensitivity to the DNA-damaging agents phleomycin and Parp1 inhibitors.
  • Research area: Cancer ; DNA Damage and Repair ; Drug Discovery & Development
  • Production details: HCT 116 BRCA2 KO cells were created through targeted disruption of human BRCA2 by homologous recombination and deletion was confirmed by Southern Blot and Western Blot using antibodies against the C-terminus of the BRCA2 protein. This cell line was noted as clone 42 by the originating laboratory.
  • Cellosaurus ID: CVCL_AS10
  • Additional notes: The originating laboratory have advised they observed no difference in culture phenotype between clone 42 and clone 46.

  • For Research Use Only

Target Details

  • Target: BRCA2

Application Details

  • Application notes: Clone 42 was generated at the same time as clone 46 cited in Xu et al., 2014. J Pathol. 2014 Nov;234(3):386-97. The originating laboratory have advised they observed no difference in culture phenotype between clone 42 and clone 46.

Handling

  • Format: Frozen
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   Xu et al. 2014. J Pathol. 234(3):386-97. PMID: 25043256.
  •   Up-regulation of the interferon-related genes in BRCA2 knockout epithelial cells.