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Contributor Information

  • Name Carlos Caldas
  • Institute Cancer Research UK, Cambridge Institute

Tool Details

  • Tool name: HCT 116 BRCA2 -/- [42] Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: HCT 116
  • Organism: Human
  • Tissue: Colon
  • Cancer type: Colon Carcinoma
  • Disease: Cancer
  • Description: BRCA2 -/- HCT116 human colorectal carcinoma cells were generated by homologous recombination to study the role of BRCA2 in DNA repair and to identify additional functions of the BRCA2 gene. These cells show phenotypes consistent with previous reports, including loss of Rad51 foci in the presence of double-strand breaks, chromosomal rearrangements and elevated sensitivity to the DNA-damaging agents phleomycin and Parp1 inhibitors.
  • Research area: Cancer ; DNA Damage and Repair ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium:
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   Xu et al. 2014. J Pathol. 234(3):386-97. PMID: 25043256.
  •   Up-regulation of the interferon-related genes in BRCA2 knockout epithelial cells.