CELL LINES
Contributor Information
- Name Carlos Caldas
- Institute Cancer Research UK Cambridge Institute
- Primary citation Xu et al. 2014. J Pathol. 234(3):386-97. PMID: 25043256
Tool Details
- Tool name: HCT 116 BRCA2 -/- [42] Cell Line
- Alternate names: HCT 116 BRCA2(-/-) clone 42; HCT 116 BRCA2(-/-)
- Clone: 42
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: HCT 116 cell line
- Organism: Human
- Tissue: Colon
- Cancer type: Digestive / Gastrointestinal cancer
- Model: Knock-Out
- Model description: Targeted disruption of human BRCA2 by homologous recombination
- CRISPR: No
- Conditional: No
- Application: Anticancer agents discovery and development; Cytotoxic agents' activity screening; Colony formation assay
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Description: BRAC2 inherited mutations predispose carriers to various early onset cancers, including breast and ovarian. HCT116 BRCA2 -/- human colorectal carcinoma cell line was generated to study the role of BRCA2 in DNA repair, investigate the effects of BRCA2 mutations in a range of cancers, and identify additional functions of the BRCA2 gene.
This homozygous knockout cell line shows phenotypes consistent with previous reports, including loss of Rad51 foci in the presence of double-strand breaks, chromosomal rearrangements and elevated sensitivity to the DNA-damaging agents phleomycin and Parp1 inhibitors - Research area: Cancer; Genetics; Drug development
- Production details: Targeted disruption of the human BRCA2 locus in HCT116 cells by homologous recombination. The gene targeting construct was generated by using a recombinant adeno-associated virus (rAAV) system. The deletion was confirmed by Southern Blot and Western Blot using antibodies against the C-terminus of the BRCA2 protein. This cell line was noted as clone 42 by the originating laboratory
- Cellosaurus ID: CVCL_AS10
- Additional notes: Clone 42 was generated at the same time as clone 46 cited in Xu et al. 2014. J Pathol. 234(3):386-97. PMID: 25043256. The originating laboratory have advised they observed no difference in culture phenotype between clone 42 and clone 46.
- For Research Use Only
Target Details
- Target: BRCA2 (BReast CAncer gene 2)
- Target background: BRCA2 is a human tumor suppressor gene found in all humans. The primary role of its protein is in HR-mediated DNA damage repair
Application Details
- Application: Anticancer agents discovery and development; Cytotoxic agents' activity screening; Colony formation assay
Handling
- Format: Frozen
- Volume: 1 ml
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Characterisation tests: BRCA2 deletion was confirmed by Southern Blot and Western Blot using antibodies against the C-terminus of the BRCA2 protein
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
Related Tools
References
- • Xu et al. 2014. J Pathol. 234(3):386-97. PMID: 25043256
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