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Contributor Information

  • Name
  • Institute National Institutes of Health

Tool Details

  • Tool name: A2780 Cell Line
  • Tool type: Cell Lines
  • Organism: Human
  • Tissue: Ovary
  • Cancer type: Gynaecologic cancer
  • Disease: Cancer
  • Growth properties: Adherent
  • Model: Tumour line
  • Conditional: Yes
  • Application: A2780 cell line is used in toxicity testing and cancer genetic studies.
  • Description: A2780 cell line used to investigate cisplatin resistance in ovarian cancer. Background and Research Application The A2780 human ovarian cancer cell line was established from tumour tissue from an untreated patient of ovarian endometroid adenocarcinoma. Cells grow as a monolayer and in suspension in spinner cultures. A2780 is the parent line to the cisplatin resistant cell line A2780 cis (catalogue no. 152708) and the adriamycin resistant cell line A2780 ADR (catalogue no. 152707). This cell line has been used in a variety of research, including understanding the role of Snail and Slug in ovarian tumour neovascularization; how to overcome cisplatin resistance in ovarian cancer cells using liposome application and studying the effect of low molecular weight heparin tinzaparin on cisplatin resistance in ovarian cancer cells. A2780 cell line is used in toxicity testing and cancer genetic studies.
  • Research area: Cancer; Drug development
  • Production details: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 3-6x10,000cells/cmÂ?‹Â› using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37Â?‚°C.
  • Cellosaurus ID: CVCL_0134
  • Additional notes: STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 12,13 D16S539: 11,13 D5S818: 11,12 D7S820: 10 THO1: 6 TPOX: 8,10 vWA: 15,16

  • For Research Use Only

Target Details

Application Details

  • Application: A2780 cell line is used in toxicity testing and cancer genetic studies.
  • Application notes: Points of Interest STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 12,13 D16S539: 11,13 D5S818: 11,12 D7S820: 10 THO1: 6 TPOX: 8,10 vWA: 15,16 Concentration Vial has between 1-5 million cells as standard, however this may vary.

Handling

  • Format: Frozen
  • Growth medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
  • Shipping conditions: Dry ice

Documentation

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References

  • •  Nusinow et al. 2020. Cell. 180(2):387-402.e16. PMID: 31978347.
  • •  Ghandi et al. 2019. Nature. 569(7757):503-508. PMID: 31068700.
  • •  Dutil et al. 2019. Cancer Res. 79(7):1263-1273. PMID: 30894373.
  • •  Zhao et al. 2017. Clin Proteomics. 14:20. PMID: 28546799.
  • •  Medrano et al. 2017. Cell Rep. 18(10):2343-2358. PMID: 28273451.
  • •  Extracellular matrix proteins expression profiling in chemoresistant variants of the A2780 ovarian cancer cell line.
  • •  Januchowski et al. 2014. Biomed Res Int. 2014:365867. PMID: 24804215.
  • •  Han et al. 2013. Oncol Lett. 6(5):1295-1298. PMID: 24179511.
  • •  A2780 human ovarian cancer cells with acquired paclitaxel resistance display cancer stem cell properties.
  • •  Barretina et al. 2012. Nature. 483(7391):603-7. PMID: 22460905.
  • •  The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
  • •  Yu et al. 2000. Int J Oncol. 16(3):555-60. PMID: 10675489.
  • •  Comparison of two human ovarian carcinoma cell lines (A2780/CP70 and MCAS) that are equally resistant to platinum, but differ at codon 118 of the ERCC1 gene.
  • •  Henkels et al. 1997. Cancer Res. 57(20):4488-92. PMID: 9377558.
  • •  Induction of apoptosis in cisplatin-sensitive and -resistant human ovarian cancer cell lines.
  • •  Parker et al. 1991. J Clin Invest. 87(3):772-7. PMID: 1999494.
  • •  Acquired cisplatin resistance in human ovarian cancer cells is associated with enhanced repair of cisplatin-DNA lesions and reduced drug accumulation.