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Contributor Information

  • Name Timothy Ward
  • Institute National Institutes of Health

Tool Details

  • Tool name: A2780ADR Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: A2780
  • Organism: Human
  • Tissue: Ovary
  • Cancer type: Carcinoma
  • Disease: Cancer
  • Growth properties: Adherent
  • Model: Tumour line
  • Conditional: Yes
  • Description: The adriamycin-resistant cell line A2780ADR has been developed by exposure of the parent A2780 cell line (catalogue no. 152706) to adriamycin. A2780ADR is cross-resistant to melphalan and vinblastine. To retain resistance adriamycin has to be added to the media. The cells grow as a monolayer and in suspension in spinner cultures and are tumourigenic in immune deficient mice. Together with the cisplatin-resistant variant A2780cis (catalogue no. 152708_ these lines only differ in their exposure to a single drug and should facilitate the search for molecular changes responsible for the expression of pleiotropic drug resistance in human ovarian cancer.
  • Research area: Cancer ; Drug Discovery & Development
  • Production details: Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Recommendation: culture cells without drug after resuscitation until growth has been fully established.
  • Cellosaurus ID: CVCL_1941
  • Additional notes: STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 13 D16S539: 11,14 D5S818: 11,12 D7S820: 10 THO1: 6 TPOX: 8,9 vWA: 15,16

  • For Research Use Only

Target Details

  • Target: Adriamycin resistance

Application Details

  • Application notes: STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 13 D16S539: 11,14 D5S818: 11,12 D7S820: 10 THO1: 6 TPOX: 8,9 vWA: 15,16

Handling

  • Format: Frozen
  • Growth medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS); treatment with 10E-7 M adriamycin at least once a week.
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Beaufort et al. 2014. PLoS One. 9(9):e103988. PMID: 25230021.
  •   Ovarian cancer cell line panel (OCCP): clinical importance of in vitro morphological subtypes.
  •   Hamilton et al. 1984. Semin Oncol. 11(3):285-98. PMID: 6385258.
  •   Experimental model systems of ovarian cancer: applications to the design and evaluation of new treatment approaches.