Cat. #152707
A2780ADR Cell Line
Cat. #: 152707
Sub-type: Continuous
Unit size: 1x10^6 cells / vial
Organism: Human
Tissue: Ovary
Disease: Cancer
Model: Tumour line
£800.00
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Contributor
Inventor: Timothy Ward
Institute: National Cancer Institute
Tool Details
*FOR RESEARCH USE ONLY
- Name: A2780ADR Cell Line
- Tool sub type: Continuous
- Parental cell: A2780
- Organism: Human
- Tissue: Ovary
- Disease: Cancer
- Growth properties: Adherent
- Model: Tumour line
- Conditional: Yes
- Description: The adriamycin-resistant cell line A2780ADR has been developed by exposure of the parent A2780 cell line (catalogue no. 152706) to adriamycin. A2780ADR is cross-resistant to melphalan and vinblastine. To retain resistance adriamycin has to be added to the media. The cells grow as a monolayer and in suspension in spinner cultures and are tumourigenic in immune deficient mice. Together with the cisplatin-resistant variant A2780cis (catalogue no. 152708_ these lines only differ in their exposure to a single drug and should facilitate the search for molecular changes responsible for the expression of pleiotropic drug resistance in human ovarian cancer.
- Production details: Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 2x10,000 cells/cmÄÂ? using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37ÄÂ?°C. Recommendation: culture cells without drug after resuscitation until growth has been fully established.
- Additional notes: STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 13 D16S539: 11,14 D5S818: 11,12 D7S820: 10 THO1: 6 TPOX: 8,9 vWA: 15,16
- Cellosaurus id: CVCL_1941
Target Details
- Target: Adriamycin resistance
Applications
- Application notes: STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 13 D16S539: 11,14 D5S818: 11,12 D7S820: 10 THO1: 6 TPOX: 8,9 vWA: 15,16
Handling
- Format: Frozen
- Growth medium: RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS); treatment with 10E-7 M adriamycin at least once a week.
- Unit size: 1x10^6 cells / vial
- Shipping conditions: Dry ice
Related Tools
- Related tools: A2780 Cell Line ; A2780cis Cell Line
References
- Beaufort et al. 2014. PLoS One. 9(9):e103988. PMID: 25230021.
- Ovarian cancer cell line panel (OCCP): clinical importance of in vitro morphological subtypes.
- Hamilton et al. 1984. Semin Oncol. 11(3):285-98. PMID: 6385258.
- Experimental model systems of ovarian cancer: applications to the design and evaluation of new treatment approaches.
