CELL LINES
Contributor Information
- Name
- Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields
Tool Details
- Tool name: CMT 93 Cell Line
- Tool type: Cell Lines
- Organism: Mouse
- Cancer type: Mouse rectum carcinoma
- Disease: Cancer
- Growth properties: Adherent
- Model: Tumour line
- Conditional: Yes
- Description: The CMT 93 cell line has been isolated from a 19 month old male mouse (C57BL/1CRF) which had received an i.p. injection of MAMA each week for 18 months. 4th in vivo passage - source of explant culture. Cells form acini and junctional complexes and produce large tumours in nude mice (6 x 1,000,000 cells inoculum) after 1 month.
- Research area: Cancer; Drug Discovery & Development
- Cellosaurus ID: CVCL_1986
- For Research Use Only
Target Details
Application Details
Handling
- Format: Frozen
- Growth medium: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cmĂ using 0.25% trypsin; 5% CO2; 37Ă°C. Cells grow in clumps. Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
- Shipping conditions: Dry ice
Documentation
- Available on request
Related Tools
References
- • Franks et al. 1978. J Pathol. 124(1):35-8. PMID: 722371.
- • A cell line from an induced carcinoma of mouse rectum.
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