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Contributor Information

  • Name
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: CMT 93 Cell Line
  • Tool type: Cell Lines
  • Parental cell line:
  • Organism: Mouse
  • Cancer type: Mouse rectum carcinoma
  • Disease: Cancer
  • Conditional: Yes
  • Description: The CMT 93 cell line has been isolated from a 19 month old male mouse (C57BL/1CRF) which had received an i.p. injection of MAMA each week for 18 months. 4th in vivo passage - source of explant culture. Cells form acini and junctional complexes and produce large tumours in nude mice (6 x 1,000,000 cells inoculum) after 1 month.
  • Research area: Cancer ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin; 5% CO2; 37°C. Cells grow in clumps. Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   Franks et al. 1978. J Pathol. 124(1):35-8. PMID: 722371.
  •   A cell line from an induced carcinoma of mouse rectum.