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Contributor Information

  • Name Nancy Hogg
  • Institute Cancer Research UK, London Research Institute: Lincoln's Inn Fields

Tool Details

  • Tool name: CHO ICAM-1Fc Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: Chinese Hamster Ovary (CHO)
  • Organism: Hamster
  • Tissue: Ovary
  • Model: Transgenic
  • Conditional: No
  • Description: CHO ICAM-1Fc cells secrete a dimeric human ICAM-1Fc protein. ICAM-1 (CD54) is a major ligand for leukocyte CD11/CD18 integrins and used in adhesion assays to evaluate integrin activity.
  • Research area: Cell biology; Immunology
  • Production details: The chimeric wild-type ICAM-1Fc protein consists of the five extracellular domains of ICAM-1 joined to the hinge, CH2 and CH3 domains of IgG1. CHO cells were transfected using DEAE-dextran and the secreted proteins were collected in serum-free medium for 4 days. The proteins were purified on a Protein Aâ?‚€?‚“Sepharose affinity column using standard methods.
  • Cellosaurus ID: CVCL_AS85

  • For Research Use Only

Target Details

  • Target: Human ICAM-1Fc chimeric protein (five extracellular domains of ICAM-1 plus hinge, CH2 and CH3 domains of IgG1)

Application Details

Handling

  • Format: Frozen
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

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References

  • •  Stanley et al. 2008. EMBO J. 27(1):62-75. PMID: 18079697.
  • •  Intermediate-affinity LFA-1 binds alpha-actinin-1 to control migration at the leading edge of the T cell.
  • •  Smith et al. 2005. J Cell Biol. 170(1):141-51. PMID: 15983060.
  • •  A talin-dependent LFA-1 focal zone is formed by rapidly migrating T lymphocytes.
  • •  Smith et al. 2003. J Cell Sci. 116(Pt 15):3123-33. PMID: 12799414.
  • •  LFA-1-induced T cell migration on ICAM-1 involves regulation of MLCK-mediated attachment and ROCK-dependent detachment.
  • •  Stanley et al. 2000. Biochem J. 351(Pt 1):79-86. PMID: 10998349.
  • •  The second domain of intercellular adhesion molecule-1 (ICAM-1) maintains the structural integrity of the leucocyte function-associated antigen-1 (LFA-1) ligand-binding site in the first domain.
  • •  Stanley et al. 1998. J Biol Chem. 273(6):3358-62. PMID: 9452454.
  • •  The I domain of integrin LFA-1 interacts with ICAM-1 domain 1 at residue Glu-34 but not Gln-73.