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Contributor Information

  • Name John Lunec
  • Institute Newcastle University

Tool Details

  • Tool name: S_N40R2 Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: SJSA-1
  • Tissue: Bone
  • Cancer type: Sarcoma
  • Disease: Cancer
  • Model: Cancer Model
  • Conditional: No
  • Description: To determine how resistance to MDM2/p53 binding antagonists might develop, SJSA-1 cells were exposed to growth inhibitory concentrations of MDM2 inhibitors, Nutlin-3, and a clonal resistant cell line generated. The p53 mediated responses of parental and resistant cell lines were compared. In contrast to the parental cell lines, p53 activation by Nutlin-3, MI-63 or ionizing radiation was not observed in the SJSA-1 derived cell lines.
  • Research area: Cancer; Apoptosis and autophagy; Cell biology; Drug development; Genetics
  • Production details: Resistant cell lines were established by exposing SJSA-1 cells to Nutlin-3. Single cell derived colonies were isolated with cloning cylinders and the clonal population expanded in culture medium containing the MDM2/p53 antagonist refreshed weekly for 60 days. Stage 1 resistant clones were then further exposed to increased concentrations of Nutlin-3 for 30 days to generate stage 2 resistant clones.
  • Cellosaurus ID: CVCL_HG10

  • For Research Use Only

Target Details

Application Details


  • Format: Frozen
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1



  •   TP53 mutant MDM2-amplified cell lines selected for resistance to MDM2-p53 binding antagonists retain sensitivity to ionizing radiation.
  •   Drummond et al. 2016. Oncotarget. :. PMID: 27323823.