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Contributor Information

  • Name John Lunec
  • Institute Newcastle University

Tool Details

  • Tool name: S_M6R1 Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: SJSA-1
  • Organism: Human
  • Tissue: Bone
  • Cancer type: Osteosarcoma; Multipotential sarcoma
  • Disease: Cancer
  • Description: To determine how resistance to MDM2/p53 binding antagonists might develop, SJSA-1 cells were exposed to growth inhibitory concentrations of a MDM2 inhibitor, MI-63, and a clonal resistant cell line was generated. The p53 mediated responses of the parental and resistant cell line were compared. In contrast to the parental cell lines, p53 activation by Nutlin-3, MI-63 or ionizing radiation was not observed in the SJSA-1 derived cell line, S-M6R1.
  • Research area: Cancer ; Apoptosis and Programmed Cell Death ; Cell Cycle ; Drug Discovery & Development ; Epigenetics & Nuclear Signalling

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium:
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   TP53 mutant MDM2-amplified cell lines selected for resistance to MDM2-p53 binding antagonists retain sensitivity to ionizing radiation.
  •   Drummond et al. 2016. Oncotarget. :. PMID: 27323823.