CELL LINES
Contributor Information
- Name John Lunec
- Institute Newcastle University
Tool Details
- Tool name: N_N20R1 Cell Line
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: NGP
- Organism: Human
- Tissue: Lung
- Cancer type: Neuroblastoma
- Disease: Cancer
- Model: Cancer Model
- Conditional: No
- Description: To determine how resistance to MDM2/p53 binding antagonists might develop, NGP cells were exposed to growth inhibitory concentrations of a MDM2 inhibitor, Nutlin-3, and a clonal resistant cell line was generated. The p53 mediated responses of the parental and resistant cell line were compared. In contrast to the parental cell lines, p53 activation by Nutlin-3, MI-63 or ionizing radiation was not observed in the NGP derived cell line, N_N20R1.
- Research area: Cancer; Apoptosis and Programmed Cell Death; Cell Cycle; Drug Discovery & Development; Epigenetics & Nuclear Signalling
- Production details: Resistant cell lines were established by exposing NGP cells to Nutlin-3. Single cell derived colonies were isolated with cloning cylinders and the clonal population expanded in culture medium containing the MDM2/p53 antagonist refreshed weekly for 60 days. Stage 1 resistant clones were then further exposed to increased concentrations of Nutlin-3 for 30 days to generate stage 2 resistant clones.
- For Research Use Only
Target Details
Application Details
Handling
- Format: Frozen
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
- Available on request
Related Tools
References
- • TP53 mutant MDM2-amplified cell lines selected for resistance to MDM2-p53 binding antagonists retain sensitivity to ionizing radiation.
- • Drummond et al. 2016. Oncotarget. :. PMID: 27323823.
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