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Contributor Information

  • Name Paul Harrison
  • Institute Cancer Research UK, Glasgow: The Beatson Institute

Tool Details

  • Tool name: D38 Cell Line
  • Tool type: Cell Lines
  • Organism: Human
  • Tissue: Tongue
  • Cancer type: Dysplasia, Leukoplakia
  • Disease: Cancer
  • Model: Tumour line
  • Conditional: No
  • Description: D38 Cell Line is derived from a leukoplakia biopsy. The cell line was notable in its proliferative capacity, considered immortal after having completed more than 100 PDs (population doublings ) when maintained on a feeder layer of irradiated 3T3 fibroblasts. D38 was categorised as having a mild dysplasia pathology.
  • Research area: Cancer ; Drug Discovery & Development
  • Production details: Biopsies were trypsinized and cultured until a growing population of cells was obtained in a 9-cm plate and then passaged once to give a stock culture that was frozen. All cells were maintained on irradiated 3T3 feeders, in 10H medium. The 3T3 feeder layer was removed by treatment with 0.02% EDTA prior to RNA and protein extraction.
  • Cellosaurus ID: CVCL_HF99

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: As per Cancer Res. 1997 Sep 15;57(18):3886-9. All cells were maintained on irradiated 3T3 feeders, in 10H medium (DMEM plus 10% FCS without added growth factors except hydrocortisone).
  • Shipping conditions: Dry ice

Documentation

  • Available on request

References

  •   McGregor et al. 2002. Cancer Res. 62(16):4757-66. PMID: 12183435.
  •   Molecular changes associated with oral dysplasia progression and acquisition of immortality: potential for its reversal by 5-azacytidine.