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Contributor Information

  • Name Francis Barr
  • Institute University of Liverpool

Tool Details

  • Tool name: HeLa EGFP-Histone H2B Cell Line
  • Alternate names: Histone H2B
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: HeLa
  • Organism: Human
  • Tissue: Cervix
  • Conditional: Yes
  • Description: The human histone H2B gene was fused to the gene encoding the enhanced green fluorescent protein (EGFP) and transfected into human HeLa cells to generate a stable line constitutively expressing H2B-GFP. The H2B-GFP fusion protein was incorporated into nucleosomes without affecting cell cycle progression. The cell line allows high-resolution imaging of both mitotic chromosomes and interphase chromatin.
  • Research area: Cell Cycle ; Cell Type or Organelle Marker ; Cell Structure and Motility ; DNA Damage and Repair ; Genetic Studies Tools

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: DMEM, 10% FBS, 5% CO2, 37°C. Antibiotic resistance for selection of GFP positive cells: 4 ?g/ml Blasticidine, expression of GFP is quite stable but selecting at least every two passages is recommended.
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   Zeng et al. 2010. J Cell Biol. 191(7):1315-32. PMID: 21187329.
  •   Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.