HeLa EGFP-Histone H2B Cell Line
- Name Francis Barr
- Institute University of Liverpool
Tool name: HeLa EGFP-Histone H2B Cell Line
Alternate names: Histone H2B
Tool type: Cell Lines
Tool sub-type: Continuous
Parental cell line: HeLa
Description: The human histone H2B gene was fused to the gene encoding the enhanced green fluorescent protein (EGFP) and transfected into human HeLa cells to generate a stable line constitutively expressing H2B-GFP. The H2B-GFP fusion protein was incorporated into nucleosomes without affecting cell cycle progression. The cell line allows high-resolution imaging of both mitotic chromosomes and interphase chromatin.
Research area: Cell Cycle ; Cell Type or Organelle Marker ; Cell Structure and Motility ; DNA Damage and Repair ; Genetic Studies Tools
- For Research Use Only
- • Zeng et al. 2010. J Cell Biol. 191(7):1315-32. PMID: 21187329.
- • Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.