CELL LINES
Contributor Information
- Name Francis Barr
- Institute University of Liverpool
Tool Details
- Tool name: HeLa EGFP-Histone H2B Cell Line
- Alternate names: Histone H2B
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: HeLa
- Organism: Human
- Tissue: Cervix
- Model: Reporter
- Conditional: Yes
- Description: The human histone H2B gene was fused to the gene encoding the enhanced green fluorescent protein (EGFP) and transfected into human HeLa cells to generate a stable line constitutively expressing H2B-GFP. The H2B-GFP fusion protein was incorporated into nucleosomes without affecting cell cycle progression. The cell line allows high-resolution imaging of both mitotic chromosomes and interphase chromatin.
- Research area: Cell biology; Genetics
- For Research Use Only
Target Details
Application Details
Handling
- Format: Frozen
- Growth medium: DMEM, 10% FBS, 5% CO2, 37?°C. Antibiotic resistance for selection of GFP positive cells: 4 ?g/ml Blasticidine, expression of GFP is quite stable but selecting at least every two passages is recommended.
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
Related Tools
References
- • Zeng et al. 2010. J Cell Biol. 191(7):1315-32. PMID: 21187329.
- • Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.
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