HeLa mCherry-Histone H2B EGFP-Alpha Tubulin Cell Line

The human histone H2B gene was fused to the gene encoding mCherry and Alpha Tubulin was similarly fused to EGFP.

Contributors

Inventor Institute
Francis Barr University of Liverpool
Cat. #: 152987
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Research Fields: Cell biology;Genetics
Organism: Human
Tissue: Cervix
Model: Reporter
Product description: The HeLa mCherry-Histone H2B EGFP-Alpha Tubulin cell line enables live-cell imaging of chromatin (both interphase and mitotic chromosomes) and microtubule dynamics. This allows for detailed study of mitosis, spindle dynamics, cytoskeletal organisation, and cellular responses to treatments. 
Production details: The human histone H2B gene was fused to the gene encoding mCherry and Alpha Tubulin was similarly fused to EGFP. Both constructs were transfected into human HeLa cells to generate a stable line constitutively expressing H2B-mCherry and EGFP-Alpha Tubulin. The mCherry-Histone H2B fusion protein was incorporated into nucleosomes without affecting cell cycle progression.
Parental cell line: HeLa S3
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM, 10% FBS, 5% CO2, 37°C. Antibiotic selection for GFP and mCherry expression: 1µg/mL Puromycin, 4µg/mL Blasticidine, expression is quite stable but selecting at least every two passages is recommended.
Recommended controls: HeLa parental line
References:

Dunsch et al. 2012. J Cell Biol. 198(6):1039-1054. PMID: 22965910.

Zeng et al. 2010. J Cell Biol. 191(7):1315-1332. PMID: 21187329.

Bastos et al. 2010. J Cell Biol. 191(4):751-760. PMID: 21079244.

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