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Contributor Information

  • Name Francis Barr
  • Institute University of Liverpool

Tool Details

  • Tool name: HeLa mCherry-Histone H2B EGFP-Alpha Tubulin Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: HeLa
  • Organism: Human
  • Tissue: Cervix
  • Model: Reporter
  • Description: The human histone H2B gene was fused to the gene encoding mCherry and Alpha Tubulin was similarly fused to EGFP. Both constructs were transfected into human HeLa cells to generate a stable line constitutively expressing H2B-mCherry and EGFP-Alpha Tubulin. The mCherry-Histone H2B fusion protein was incorporated into nucleosomes without affecting cell cycle progression. The cell line allows high-resolution imaging of both mitotic chromosomes and interphase chromatin.
  • Research area: Cell biology; Genetics

  • For Research Use Only

Target Details

  • Target: Histone H2B, Alpha Tubulin

Application Details

Handling

  • Format: Frozen
  • Growth medium: DMEM, 10% FBS, 5% CO2, 37?‚°C. Antibiotic selection for GFP and mCherry expression: 1?g/mL Puromycin, 2?g/mL Blasticidine, expression is quite stable but selecting at least every two passages is recommended.
  • Shipping conditions: Dry ice

Documentation

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References

  • •  Dunsch et al. 2012. J Cell Biol. 198(6):1039-54. PMID: 22965910.
  • •  Dynein light chain 1 and a spindle-associated adaptor promote dynein asymmetry and spindle orientation.
  • •  Zeng et al. 2010. J Cell Biol. 191(7):1315-32. PMID: 21187329.
  • •  Protein phosphatase 6 regulates mitotic spindle formation by controlling the T-loop phosphorylation state of Aurora A bound to its activator TPX2.
  • •  Bastos et al. 2010. J Cell Biol. 191(4):751-60. PMID: 21079244.
  • •  Plk1 negatively regulates Cep55 recruitment to the midbody to ensure orderly abscission.