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Contributor Information

  • Name Joy Burchell ; Joyce Taylor-Papadimitriou
  • Institute King's College London

Tool Details

  • Tool name: T47D-E2J Cell Line
  • Alternate names: C2GnT C2GnT-L
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: T47D
  • Organism: Human
  • Tissue: Breast
  • Cancer type: Breast cancer
  • Disease: Cancer
  • Conditional: No
  • Description: The parental T47D cell line was established from the pleural effusion of a ductal carcinoma of the breast. The tumour cell line, T47D-E2J, was created as a breast cancer model with an O-linked glycosylation profile similar to normal mammary epithelial cells. T47D-E2J cells overexpress the enzyme C2GnT1, a beta-1,3galactosyl-O-glycosyl-glycoprotein. C2GnT1 is the dominant enzyme expressed in normal breast tissue known to convert core 1 glycoproteins to core 2 glycoproteins, by the addition of N-acetylglucosamine to N-acetylgalactosamine. Expression of the C2GnT1 enzyme has been found to be decreased or absent in most breast cancer cell lines despite still expressing mRNAs coding for this enzyme. Together with wild-type T47D and T47D-STn these cell lines represent a panel of isogenic cells with different O-linked glycosylation patterns.
  • Research area: Cancer; Drug development

  • For Research Use Only

Target Details

  • Target: Glycosyltransferase - C2GnT1

Application Details

Handling

  • Format: Frozen
  • Growth medium: DMEM 10% FCS and 500?„?žg/ml G418
  • Shipping conditions: Dry ice

Documentation

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References

  • •  Sewell et al. 2006. J Biol Chem. 281(6):3586-94. PMID: 16319059.
  • •  The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer.
  • •  Dalziel et al. 2001. J Biol Chem. 276(14):11007-15. PMID: 11118434.
  • •  The relative activities of the C2GnT1 and ST3Gal-I glycosyltransferases determine O-glycan structure and expression of a tumor-associated epitope on MUC1.