T47D-E2J Cell Line
- Name Joy Burchell ; Joyce Taylor-Papadimitriou
- Institute King's College London
Tool name: T47D-E2J Cell Line
Alternate names: C2GnT
Tool type: Cell Lines
Tool sub-type: Continuous
Parental cell line: T47D
Cancer type: Breast cancer
Description: The parental T47D cell line was established from the pleural effusion of a ductal carcinoma of the breast. The tumour cell line, T47D-E2J, was created as a breast cancer model with an O-linked glycosylation profile similar to normal mammary epithelial cells. T47D-E2J cells overexpress the enzyme C2GnT1, a beta-1,3galactosyl-O-glycosyl-glycoprotein.
C2GnT1 is the dominant enzyme expressed in normal breast tissue known to convert core 1 glycoproteins to core 2 glycoproteins, by the addition of N-acetylglucosamine to N-acetylgalactosamine. Expression of the C2GnT1 enzyme has been found to be decreased or absent in most breast cancer cell lines despite still expressing mRNAs coding for this enzyme.
Together with wild-type T47D and T47D-STn these cell lines represent a panel of isogenic cells with different O-linked glycosylation patterns.
Research area: Cancer; Drug development
- For Research Use Only
- • Sewell et al. 2006. J Biol Chem. 281(6):3586-94. PMID: 16319059.
- • The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer.
- • Dalziel et al. 2001. J Biol Chem. 276(14):11007-15. PMID: 11118434.
- • The relative activities of the C2GnT1 and ST3Gal-I glycosyltransferases determine O-glycan structure and expression of a tumor-associated epitope on MUC1.