T47D-E2J Cell Line

C2GnT1 is the dominant enzyme expressed in normal breast tissue known to convert core 1 glycoproteins to core 2 glycoproteins, by the addition of N-acetylglucosamine to N-acetylgalactosamine. Expression of the C2GnT1 enzyme has been found to be decreased or absent in most breast cancer cell lines despite still expressing mRNAs coding for this enzyme.

Contributors

Inventor Institute
Joy Burchell ; Joyce Taylor-Papadimitriou King's College London
Cat. #: 153169
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Breast cancer
Research Fields: Cancer;Drug development
Organism: Human
Tissue: Breast
Cancer Types In Detail: Breast cancer
Alternate name: C2GnTC2GnT-L
Product description: The parental T47D cell line was established from the pleural effusion of a ductal carcinoma of the breast. The tumour cell line, T47D-E2J, was created as a breast cancer model with an O-linked glycosylation profile similar to normal mammary epithelial cells. T47D-E2J cells overexpress the enzyme C2GnT1, a beta-1,3galactosyl-O-glycosyl-glycoprotein.C2GnT1 is the dominant enzyme expressed in normal breast tissue known to convert core 1 glycoproteins to core 2 glycoproteins, by the addition of N-acetylglucosamine to N-acetylgalactosamine. Expression of the C2GnT1 enzyme has been found to be decreased or absent in most breast cancer cell lines despite still expressing mRNAs coding for this enzyme. Together with wild-type T47D and T47D-STn these cell lines represent a panel of isogenic cells with different O-linked glycosylation patterns.
Conditional: No
Parental cell line: T47D
Disease: Cancer
Format: Frozen
Shipping conditions: Dry ice
Growth medium: DMEM 10% FCS and 500?„?žg/ml G418
References:

Sewell et al. 2006. J Biol Chem. 281(6):3586-94. PMID: 16319059.

The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer.

Dalziel et al. 2001. J Biol Chem. 276(14):11007-15. PMID: 11118434.

The relative activities of the C2GnT1 and ST3Gal-I glycosyltransferases determine O-glycan structure and expression of a tumor-associated epitope on MUC1.

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