CELL LINES
Contributor Information
- Name Joy Burchell ; Joyce Taylor-Papadimitriou
- Institute King's College London
Tool Details
- Tool name: T47D-STn Cell Line
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: T47D
- Organism: Human
- Tissue: Breast
- Cancer type: Breast cancer
- Disease: Cancer
- Conditional: No
- Description: The parental T47D cell line was established from the pleural effusion of a ductal carcinoma of the breast. The tumour cell line, T47D-STn, was created as a breast cancer model which expresses the Sialyl-Tn, STn, antigen. T47D-STn cells overexpress the enzymes C2GnT1, a beta-1,3galactosyl-O-glycosyl-glycoprotein and glycosyltransferase, ST6GalNAc-I. ST6GalNAc-I which is found throughout the Golgi, can catalyse the transfer of sialic acid to N-acetylgalactosamine and is predominately limited to the gastrointestinal tract in normal adult tissues. Expression of ST6GalNAc-I in human cells promotes formation of the STn antigen which is a saccharide structure (N-acetylgalactosamine) linked to serine or threonine by a glycosidic bond. STn antigens are not usually found on healthy cell surfaces but often on human breast cancer cells. Together with wild-type T47D and T47D-E2J these cell lines represent a panel of isogenic cells with different O-linked glycosylation patterns.
- Research area: Cancer; Drug development
- For Research Use Only
Target Details
- Target: N-acetylgalactosaminide alpha-2,6-sialyltransferase 1 - ST6GalNAc-1
Application Details
Handling
- Format: Frozen
- Growth medium: DMEM 10% FCS, 500 ?g/ml G418, 300 ?g/ml hygromycin
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
Related Tools
References
- • Sewell et al. 2006. J Biol Chem. 281(6):3586-94. PMID: 16319059.
- • The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer.
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