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Contributor Information

  • Name Joy Burchell ; Joyce Taylor-Papadimitriou
  • Institute King's College London

Tool Details

  • Tool name: T47D-STn Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: T47D
  • Organism: Human
  • Tissue: Breast
  • Cancer type: Breast Cancer
  • Disease: Cancer
  • Description: The parental T47D cell line was established from the pleural effusion of a ductal carcinoma of the breast. The tumour cell line, T47D-STn, was created as a breast cancer model which expresses the Sialyl-Tn, STn, antigen. T47D-STn cells overexpress the enzymes C2GnT1, a beta-1,3galactosyl-O-glycosyl-glycoprotein and glycosyltransferase, ST6GalNAc-I. ST6GalNAc-I which is found throughout the Golgi, can catalyse the transfer of sialic acid to N-acetylgalactosamine and is predominately limited to the gastrointestinal tract in normal adult tissues. Expression of ST6GalNAc-I in human cells promotes formation of the STn antigen which is a saccharide structure (N-acetylgalactosamine) linked to serine or threonine by a glycosidic bond. STn antigens are not usually found on healthy cell surfaces but often on human breast cancer cells. Together with wild-type T47D and T47D-E2J these cell lines represent a panel of isogenic cells with different O-linked glycosylation patterns.
  • Research area: Cancer ; Drug Discovery & Development

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: DMEM 10% FCS, 500 ?g/ml G418, 300 ?g/ml hygromycin
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

  • Available on request

References

  •   Sewell et al. 2006. J Biol Chem. 281(6):3586-94. PMID: 16319059.
  •   The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer.