T47D-STn Cell Line
- Name Joy Burchell ; Joyce Taylor-Papadimitriou
- Institute King's College London
Tool name: T47D-STn Cell Line
Tool type: Cell Lines
Tool sub-type: Continuous
Parental cell line: T47D
Cancer type: Breast Cancer
Description: The parental T47D cell line was established from the pleural effusion of a ductal carcinoma of the breast. The tumour cell line, T47D-STn, was created as a breast cancer model which expresses the Sialyl-Tn, STn, antigen.
T47D-STn cells overexpress the enzymes C2GnT1, a beta-1,3galactosyl-O-glycosyl-glycoprotein and glycosyltransferase, ST6GalNAc-I.
ST6GalNAc-I which is found throughout the Golgi, can catalyse the transfer of sialic acid to N-acetylgalactosamine and is predominately limited to the gastrointestinal tract in normal adult tissues. Expression of ST6GalNAc-I in human cells promotes formation of the STn antigen which is a saccharide structure (N-acetylgalactosamine) linked to serine or threonine by a glycosidic bond. STn antigens are not usually found on healthy cell surfaces but often on human breast cancer cells.
Together with wild-type T47D and T47D-E2J these cell lines represent a panel of isogenic cells with different O-linked glycosylation patterns.
Research area: Cancer ; Drug Discovery & Development
- For Research Use Only
- • Sewell et al. 2006. J Biol Chem. 281(6):3586-94. PMID: 16319059.
- • The ST6GalNAc-I sialyltransferase localizes throughout the Golgi and is responsible for the synthesis of the tumor-associated sialyl-Tn O-glycan in human breast cancer.