HCT 116 p300 KO [F2] Cell Line

Exclusive Tool

The HCT 116 p300 KO Cell Line is a tool for the in vitro study of p300 in colon carcinoma, and specifically of its role in p53-dependent apoptosis, cellular adhesion and migration.

Contributors

Inventor Institute
Carlos Caldas Cancer Research UK Cambridge Institute
SKU: 153185
Product description: The HCT 116 p300 KO Cell Line is a tool for the in vitro study of p300 in colon carcinoma, and specifically of its role in p53-dependent apoptosis, cellular adhesion and migration. Cell lines available are p300 WT (HCT116), p300 KO Clone D10 (HCT116), p300 KO Clone F5 (HCT116), p300 KO Clone F2 (HCT116). Experiments conducted should be using at least 2 of the 3 KO cell lines. The best 2 KO clones are D10 and F5. Anyone requesting the cell line should use the parental cell line that the KOs were generated from. The p300 KO lines demonstrate an aggressive cancer phenotype in vitro, with loss of cell-cell adhesion, defects in cell-matrix adhesion, and increased migration through collagen matrices. p300 is a transcriptional cofactor involved in regulating multiple cellular processes including cell cycle regulation, proliferation, differentiation, apoptosis, DNA damage repair and adhesion properties. Somatic inactivating mutations of p300 are associated with several cancers including breast, colorectal and gastric cancers. CBP was not manipulated in this cell line
Cellosaurus ID: CVCL_HG28
Parental cell line: HCT 116
Disease: Cancer
Cat. #: 153185
Provenance and Ownership: The HCT 116 p300 KO [F2] Cell Line is owned by Cancer Research UK. CancerTools supplies the HCT 116 p300 KO [F2] Cell Line under licence from Cancer Research UK and is the authorised source for its distribution. Sourcing HCT 116 p300 KO [F2] Cell Line directly from the IP owner ensures authenticity, provenance and compliance with all applicable licensing and research use requirements.
Tool sub type: Continuous
Unit size: 1×10^6 cells / vial
Cancer types: Colorectal cancer
Research Fields: Cancer; Cell biology; Drug development
Organism: Human
Tissue: Colon
Model: Knock-Out
Growth properties: Adhesion properties; invasion; migration
Cancer Types In Detail: Carcinoma
Format: Frozen
Shipping conditions: Dry ice
Growth medium: McCoy’s 5A medium + 10% FCS
References:

?-HPV 5 and 8 E6 disrupt homology dependent double strand break repair by attenuating BRCA1 and BRCA2 expression and foci formation.

Wallace et al. 2015. PLoS Pathog. 11(3):e1004687. PMID: 25803638.

Iyer et al. 2007. Oncogene. 26(1):21-9. PMID: 16878158.

Bundy et al. 2006. Cancer Res. 66(15):7606-14. PMID: 16885360.

Metabolic consequences of p300 gene deletion in human colon cancer cells.

p300 is required for orderly G1/S transition in human cancer cells.

Krubasik et al. 2006. Br …

Images

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HCT 116 p300 KO [F2] Cell Line. Early log phase. Image courtesy of the European Collection of Authenticated Cell Cultures (ECACC)

Related Tools for Cell lines

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153184 HCT 116 p300 KO [F5] Cell Line Key Info View Tool
151479 HCT 116 p300 KO [D10] Cell Line Key Info View Tool
153183 HCT 116 p300 WT Cell Line Key Info View Tool
153186 HCT 116 p300 KO Rescue Cell Line Key Info View Tool

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