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Contributor Information

  • Name Sandra Van Schaeybroeck
  • Institute Queen's University Belfast

Tool Details

  • Tool name: HCT 116 AXL KO Tet-inducible Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: HCT 116
  • Organism: Human
  • Tissue: Colon
  • Cancer type: Digestive / Gastrointestinal cancer
  • Disease: Cancer
  • Growth properties: Invasion, migration
  • Model: Knock-Out
  • Conditional: Yes
  • Conditional description: Conditional knockdown of endogenous AXL expression upon treatment with Tetracycline
  • Description: Receptor tyrosine kinase (RTK) screens identifed AXL as a protein which underpins the migratory/invasive phenotype in colorectal cancer (CRC) (Dunne et al., 2014). AXL was shown to be a poor prognostic marker and an important mediator of cell migration/invasiveness. The HCT 116 AXL KO Tet-inducible cell line enables further investigation into the target as a prognostic biomarker and therapeutic target.
  • Research area: Cancer; Cell biology; Drug development
  • Production details: HCT 116 cells were transfected with pTRIPZ plasmid encoding Tet-inducible shRNA against AXL. Stably transfected cells were selected, maintained in mediumsupplemented with 0.5ÎĚ?Ÿg/mL puromycin and induced with 2ÎĚ?Ÿg/ml doxycycline.
  • Cellosaurus ID: CVCL_HG04

  • For Research Use Only

Target Details

  • Target: AXL

Application Details


  • Format: Frozen
  • Growth medium: McCoy's 5a Medium (GIBCO # 16600) + 10% FBS + 100 units/ml penicillin+ 100 ?g/ml streptomycin
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1



  •   Dunne et al. 2014. Clin Cancer Res. 20(1):164-75. PMID: 24170546.
  •   AXL is a key regulator of inherent and chemotherapy-induced invasion and predicts a poor clinical outcome in early-stage colon cancer.