CELL LINES
Contributor Information
- Name Sandra Van Schaeybroeck
- Institute Queen's University Belfast
Tool Details
- Tool name: HCT 116 AXL KO Tet-inducible Cell Line
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: HCT 116
- Organism: Human
- Tissue: Colon
- Cancer type: Digestive / Gastrointestinal cancer
- Disease: Cancer
- Growth properties: Invasion, migration
- Model: Knock-Out
- Conditional: Yes
- Conditional description: Conditional knockdown of endogenous AXL expression upon treatment with Tetracycline
- Description: Receptor tyrosine kinase (RTK) screens identifed AXL as a protein which underpins the migratory/invasive phenotype in colorectal cancer (CRC) (Dunne et al., 2014). AXL was shown to be a poor prognostic marker and an important mediator of cell migration/invasiveness. The HCT 116 AXL KO Tet-inducible cell line enables further investigation into the target as a prognostic biomarker and therapeutic target.
- Research area: Cancer; Cell biology; Drug development
- Production details: HCT 116 cells were transfected with pTRIPZ plasmid encoding Tet-inducible shRNA against AXL. Stably transfected cells were selected, maintained in mediumsupplemented with 0.5ÄÂÄ?g/mL puromycin and induced with 2ÄÂÄ?g/ml doxycycline.
- Cellosaurus ID: CVCL_HG04
- For Research Use Only
Target Details
- Target: AXL
Application Details
Handling
- Format: Frozen
- Growth medium: McCoy's 5a Medium (GIBCO # 16600) + 10% FBS + 100 units/ml penicillin+ 100 ?g/ml streptomycin
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
Related Tools
References
- • Dunne et al. 2014. Clin Cancer Res. 20(1):164-75. PMID: 24170546.
- • AXL is a key regulator of inherent and chemotherapy-induced invasion and predicts a poor clinical outcome in early-stage colon cancer.
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