CELL LINES
Contributor Information
- Name Sandra Van Schaeybroeck
- Institute Queen's University Belfast
Tool Details
- Tool name: HCT 116 EphA2 KO Tet-inducible Cell Line
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: HCT 116
- Organism: Human
- Tissue: Colon
- Cancer type: Colorectal
- Disease: Cancer
- Growth properties: Invasion, migration
- Model: Knock-Out
- Conditional: Yes
- Conditional description: Conditional knockdown of endogenous EphA2 expression upon treatment with Tetracycline
- Description: EphA2 is an important regulator of tumour initiation, neo-vascularization and metastasis in a wide range of cancers. The HCT 116 EphA2 KO Tet-inducible cell line was developed to address the involvement of EphA2 where it was shown that silencing of the protein suppressed migration and invasion.
- Research area: Cancer; Cell Structure and Motility; Drug Discovery & Development
- Production details: HCT 116 cells were transfected with pTRIPZ plasmid encoding Tet-inducible shRNA against EphA2. Stably transfected cells were selected, maintained in mediumsupplemented with 0.5ÄÂÄšĹg/mL puromycin and induced with 2ÄÂÄšĹg/ml doxycycline.
- Cellosaurus ID: CVCL_HG05
- For Research Use Only
Target Details
- Target: EphA2
Application Details
Handling
- Format: Frozen
- Growth medium: McCoy's 5a Medium (GIBCO # 16600) + 10% FBS + 100 units/ml penicillin+ 100 ?g/ml streptomycin
- Shipping conditions: Dry ice
Documentation
- Available on request
Related Tools
References
- • Dunne et al. 2016. Clin Cancer Res. 22(1):230-42. PMID: 26283684.
- • EphA2 Expression Is a Key Driver of Migration and Invasion and a Poor Prognostic Marker in Colorectal Cancer.
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