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Contributor Information

  • Name Sandra Van Schaeybroeck
  • Institute Queen's University Belfast

Tool Details

  • Tool name: HCT 116 EphA2 KO Tet-inducible Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: HCT 116
  • Organism: Human
  • Tissue: Colon
  • Cancer type: Colorectal
  • Disease: Cancer
  • Growth properties: Invasion, migration
  • Model: Knock-Out
  • Conditional: Yes
  • Conditional description: Conditional knockdown of endogenous EphA2 expression upon treatment with Tetracycline
  • Description: EphA2 is an important regulator of tumour initiation, neo-vascularization and metastasis in a wide range of cancers. The HCT 116 EphA2 KO Tet-inducible cell line was developed to address the involvement of EphA2 where it was shown that silencing of the protein suppressed migration and invasion.
  • Research area: Cancer; Cell Structure and Motility; Drug Discovery & Development
  • Production details: HCT 116 cells were transfected with pTRIPZ plasmid encoding Tet-inducible shRNA against EphA2. Stably transfected cells were selected, maintained in mediumsupplemented with 0.5Îźg/mL puromycin and induced with 2Îźg/ml doxycycline.
  • Cellosaurus ID: CVCL_HG05

  • For Research Use Only

Target Details

  • Target: EphA2

Application Details


  • Format: Frozen
  • Growth medium: McCoy's 5a Medium (GIBCO # 16600) + 10% FBS + 100 units/ml penicillin+ 100 ?g/ml streptomycin
  • Shipping conditions: Dry ice


  • Available on request


  •   Dunne et al. 2016. Clin Cancer Res. 22(1):230-42. PMID: 26283684.
  •   EphA2 Expression Is a Key Driver of Migration and Invasion and a Poor Prognostic Marker in Colorectal Cancer.